Antibody‐DNA oligo conjugates are enabling a new era of single cell analysis. Single-cell multiomics now allow for simultaneous profiling of RNA and protein from single cells. CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) and similar methods seamlessly integrate antibody-oligo conjugates with existing single-cell sequencing workflows, allowing antibody-directed quantification of cellular proteins simultaneously with the RNA transcriptome.

BioLegend’s revolutionary TotalSeq™ oligo-conjugated antibodies provide a standardized process for antibody-directed protein characterization. This allows for measurement of cell surface proteins without the multiplexing limitation of fluorophores or heavy metals. To date we have successfully mutiplexed more than 220 proteins. TotalSeq™ Cell Hashing antibodies also facilitate sample multiplexing by targeting ubiquitously-expressed surface proteins. TotalSeq™ Cell Hashing antibodies combined with antibody-directed protein analysis enable extensive sample data cells incorporating targets from both the proteome and transcriptome of single cells.

Join us to review the use of TotalSeq™ antibodies in exciting applications such as CITE-seq, and how they interface with commercial scRNAseq solutions, such as the 10X Genomics system with Feature Barcoding Technology™. We will discuss technical considerations, collaborator data, new products in development, and more.

BioLegend Seminar: TotalSeq™, Revolutionary Highly Multiplexed Protein Analysis in Single-Cell Multi-Omics

Date: July 16, 2020 at 12 pm EST

Speaker: John Rutigliano, Ph.D., Technical Application Scientist II, BioLegend Inc.