With wide varieties of antibodies, fluorophores, and instruments available for multicolor flow cytometry, optimizing your multicolor experiments can be confusing and frustrating. The task of increasing the number of markers in a multicolor panel requires that a set of guiding principles be followed, to ensure that sensitivity and statistical consistency are maintained between sample and day. As the number of markers increases, and thus the number of colors with overlapping spectra increases, so do the complexities of experimentation and analysis. You can minimize aberrations associated with poor assay planning, lack of prior antibody titration optimization, and inadequate use of appropriate controls to ensure confidence in the resulting data set. Here, BioLegend offers a multitude of web tools that can assist you in planning, developing and optimizing your flow cytometry experiments.

BioLegend Seminar: Flow 101: Multi-Color Flow Cytometric Panel Design and Considerations

Date: Wednesday, July 15^th, 11:00 AM CST

Speaker: Kim Cardenas, PhD