In the human body, billions of cells die every day; this cell death is termed apoptosis. Clearance of these cells is crucial for the maintenance of homeostasis, and its dysregulation can lead to inflammatory diseases and cancer. However, many optical probes for the detection of apoptotic cells are incompatible with physiological conditions and in vivo assays. In this webinar, Dr. Nicole Barth and Prof. Marc Vendrell of the University of Edinburgh will describe how Apotracker™ Green (also known as Apo-15 peptide) was optimized to detect and image apoptosis in real time. Apotracker™ Green is a highly stable fluorogenic peptide that selectively stains apoptotic cells in a calcium-independent manner and under wash-free conditions. They will discuss their approach to identify its molecular target and this marker for fluorescence-based assays, including in vitro assays and in vivo mouse models of lung inflammation and breast cancer.
What you will learn:
- How Apotracker™ Green was designed, optimized, and validated
- Advantages and limitations compared with other reagents for detection and imaging of apoptotic cells
- How to choose the best reagents to detect apoptosis