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Overview and References

TotalSeq™ Reagents for Single-Cell Protein and RNA Detection


TotalSeq™ oligo-conjugated antibodies enable measurement of proteins at a single-cell level and integrate seamlessly into existing single-cell RNA sequencing workflows, including Drop-Seq and those available from 10x Genomics.


Simultaneous Multiomic Data Generation: Increase the power of single-cell experiments by combining proteomic and transcriptomic data.

Reduced Dropouts: In contrast to mRNA, TotalSeq™-derived antibody tags are not highly prone to dropouts, which are essentially false negative readouts.
 

Ultra-High Parameter Surface and Intracellular Protein Detection: Detect hundreds of proteins in a single cell.  A lower dropout rate contributes to enhanced sample clustering and the ability to better identify specific cell types.

 

Diverse applications: With a wide-range of mouse and human targets available, you can use TotalSeq™ antibodies in a variety of research areas, including:

  • Personalized or Precision Medicine
  • Cancer Research
  • Stem Cell Research
  • Basic and Applied Immunology
  • Biomarker Discovery
  • Characterization of New or Rare Cell Types
  • Neuroimmunology
  • Vaccine Research
 
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

Explore Additional Resources

 

Visit our 10x Genomics partnership page to learn how our TotalSeq reagents integrate with their single-cell solutions. 

 

Access our optimized protocols for staining of surface and intracellular proteins specifically developed for use with our TotalSeq reagents for CITE-Seq applications. Explore our full list of TotalSeq antibodies for surface proteins and intracellular proteins. 

 

For more information regarding the development of the CITE-Seq methodology, check out the CITE-Seq website.  

 

Discover how our TotalSeq™ reagents and recombinant proteins can be used to build antigen multimers to characterize B cell response to infection with LIBRA-seq.

Highlighted Publications

 

Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19

Su Y. et al. Cell. 2020; October. DOI: 10.1016/j.cell.2020.10.037

 

A major study aimed at performing multiomic analyses to characterize immune responses from COVID-19 patients. Using a panel of over 190 TotalSeq™ antibodies, this study identified novel immune cell populations that emerge in patients experiencing moderate COVID-19 severity that are expanded in severe cases. Additionally, the study identified changes within plasma metabolite profiles of patients experiencing severe COVID-19 disease that indicate that depletion of certain metabolites is correlated with the development of severe disease.

 

 

A Conserved Dendritic Cell Regulatory Program Limits Antitumour Immunity

Maier B, et al. Nature. 2020; doi: 10.1038/s41586-020-2134-y.

 

Immune checkpoint blockade is a recent cancer treatment method that induces a durable antitumor response. This therapy aims to mitigate the tolerogenic immune response induced by dendritic cells upon presentation of tumor antigen to T cells. However, it is only effective in a limited subset of cancer patients. This study aims to understand the mechanism by which enhancement of systemic antitumor T cell immunity occurs after neoadjuvant PD-L1 blockade. Through the use of single-cell proteogenomics, the authors identify a dendritic cell regulatory program that limits antitumor activity and is induced by expression of IL-4. Blockade of IL-4 results in increased antitumor responses, suggesting a potential treatment for the improvement of immune checkpoint therapy. 

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