Conjugation of antibodies to oligonucleotides has many applications, from their potential utility as adjuvants or targeted drug delivery systems, to the recently published simultaneous protein and RNA quantification in single cells, these reagents are opening new avenues to enable researchers move forward the frontiers of science.


A recent publication by Stoeckius et al. used antibodies coupled with oligonucleotides to simultaneously measure proteins and RNA at a single cell level. The method, termed cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), combines highly multiplexed protein marker detection with unbiased transcriptome profiling for thousands of single cells. The authors also report that the method is compatible with two high-throughput scRNA-seq platforms, Drop-seq and 10X Genomics. A similar approach was used by Peterson et al., they termed their workflow RNA expression and protein sequencing assay (REAP-seq).

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Originally, Stoeckius et al. used antibodies conjugated to streptavidin and biotinylated oligonucleotides to link the antibody and the oligonucleotide together. BioLegend is now offering antibodies directly conjugated to oligonucleotides, under our brand name TotalSeq™, to help accelerate single cell genomics and other applications.


BioLegend’s revolutionary TotalSeq™ product suite integrates seamlessly with these workflows, and enables highly multiplexed proteomics and proteogenomics across a range of next generation sequencing applications, from bulk sample analysis to highly parallel single cell sequencing. Read the press releases announcing BioLegend and New York Genome Center® exclusive global license and research agreement, as well as our collaboration with 10x Genomics. To view the list of already assigned barcodes, please click in the Barcodes tab. To request a custom conjugated antibody, please fill in the Custom Order Form.

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