In addition to our collection of antibody oligonucleotide conjugates for protein quantification, we are also refining the process of identifying each cell with its sample origin, as well as data generated from doublets instead of single cells when mixing multiple samples. The method is powered by our cell hashtag reagents. Please read Stoeckius M. et al. for more information about cell hashing.

 

The hashtags are designed so that they are specific for human or mouse cells, and to cover as many cells as possible. For human samples the hashtags are made of two antibodies that recognize ubiquitous surface markers, CD298 and β2 microglobulin, each conjugated to the same oligonucleotide containing the barcode sequence. For mouse samples, the surface markers are CD45 and H-2 MHC class I. The conjugates are already pre-mixed and ready to use.

 

For TotalSeq™-A reagents, the PCR handle between regular antibodies and the hashtag versions is different. In practice, this means that two different libraries can be prepared, one for the regular antibodies (commonly referred as ADT), and one for the hashtags (commonly referred to as HTO). They can then be mixed at different ratios before sequencing, to avoid an excess of HTO readings.

 

For TotalSeq™-C reagents, the PCR handle and capture sequence between regular antibodies and the hashtag versions is the same. In practice, this means that preparation of two different libraries is no longer possible. Thus, the most efficient way to avoid an excess of HTO readings is to titrate the Hashtag reagents. There are several ways to titrate TotalSeq™ reagents. Please read the FAQs for more information.

The tables below contains barcodes for products available to purchase, as well as conjugates that will released in the future.

 

Click on the links below to jump to a table.

TotalSeq™-A

TotalSeq™-C

TotalSeq™-A

Download as Excel document:
Category Barcode Specificity Reactivity Barcode sequence
TotalSeq™-A 0251 anti-human Hashtag 1 Human GTCAACTCTTTAGCG
TotalSeq™-A 0252 anti-human Hashtag 2 Human TGATGGCCTATTGGG
TotalSeq™-A 0253 anti-human Hashtag 3 Human TTCCGCCTCTCTTTG
TotalSeq™-A 0254 anti-human Hashtag 4 Human AGTAAGTTCAGCGTA
TotalSeq™-A 0255 anti-human Hashtag 5 Human AAGTATCGTTTCGCA
TotalSeq™-A 0256 anti-human Hashtag 6 Human GGTTGCCAGATGTCA
TotalSeq™-A 0257 anti-human Hashtag 7 Human TGTCTTTCCTGCCAG
TotalSeq™-A 0258 anti-human Hashtag 8 Human CTCCTCTGCAATTAC
TotalSeq™-A 0259 anti-human Hashtag 9 Human CAGTAGTCACGGTCA
TotalSeq™-A 0260 anti-human Hashtag 10 Human ATTGACCCGCGTTAG
TotalSeq™-A 0262 anti-human Hashtag 12 Human TAACGACCAGCCATA
TotalSeq™-A 0263 anti-human Hashtag 13 Human AAATCTCTCAGGCTC
TotalSeq™-A 0264 anti-human Hashtag 14 Human CTGTATGTCCGATTG
TotalSeq™-A 0265 anti-human Hashtag 15 Human TAAGATTCAGAGCGA
TotalSeq™-A 0266 anti-Biotin Hashtag 1 Biotin GGTAGTCATCGTGGT
TotalSeq™-A 0267 anti-Biotin Hashtag 2 Biotin GTCCGTATTCTGTCA
TotalSeq™-A 0268 anti-Biotin Hashtag 3 Biotin TCCCTTTATGCGAGG
TotalSeq™-A 0269 anti-Biotin Hashtag 4 Biotin AAAGCATGACCAGTA
TotalSeq™-A 0270 anti-Biotin Hashtag 5 Biotin TACAGTCGCTTGGCA
TotalSeq™-A 0271 anti-Biotin Hashtag 6 Biotin CTCTCCCTTTGTAGT
TotalSeq™-A 0272 anti-Biotin Hashtag 7 Biotin GGGCTTATCATTAAG
TotalSeq™-A 0274 anti-Biotin Hashtag 9 Biotin TGGAGTATCACCAAT
TotalSeq™-A 0275 anti-Biotin Hashtag 10 Biotin AAGAACGACCTCCTT
TotalSeq™-A 0301 anti-mouse Hashtag 1 Mouse ACCCACCAGTAAGAC
TotalSeq™-A 0302 anti-mouse Hashtag 2 Mouse GGTCGAGAGCATTCA
TotalSeq™-A 0303 anti-mouse Hashtag 3 Mouse CTTGCCGCATGTCAT
TotalSeq™-A 0304 anti-mouse Hashtag 4 Mouse AAAGCATTCTTCACG
TotalSeq™-A 0305 anti-mouse Hashtag 5 Mouse CTTTGTCTTTGTGAG
TotalSeq™-A 0306 anti-mouse Hashtag 6 Mouse TATGCTGCCACGGTA
TotalSeq™-A 0307 anti-mouse Hashtag 7 Mouse GAGTCTGCCAGTATC
TotalSeq™-A 0308 anti-mouse Hashtag 8 Mouse TATAGAACGCCAGGC
TotalSeq™-A 0309 anti-mouse Hashtag 9 Mouse TGCCTATGAAACAAG
TotalSeq™-A 0310 anti-mouse Hashtag 10 Mouse CCGATTGTAACAGAC
TotalSeq™-A 0311 anti-mouse Hashtag 11 Mouse GCTTACCGAATTAAC
TotalSeq™-A 0312 anti-mouse Hashtag 12 Mouse CTGCAAATATAACGG
TotalSeq™-A 0313 anti-mouse Hashtag 13 Mouse CTACATTGCGATTTG
TotalSeq™-A 0314 anti-mouse Hashtag 14 Mouse CTTTCGCCAACTCTG
TotalSeq™-A 0315 anti-mouse Hashtag 15 Mouse CCCTCTCTGGATTCT

No Results Found. Please try a different search term.

TotalSeq™-C

Download as Excel document:
Category Barcode Specificity Reactivity Barcode sequence
TotalSeq™-C 0251 anti-human Hashtag 1 Human GTCAACTCTTTAGCG
TotalSeq™-C 0252 anti-human Hashtag 2 Human TGATGGCCTATTGGG
TotalSeq™-C 0253 anti-human Hashtag 3 Human TTCCGCCTCTCTTTG
TotalSeq™-C 0254 anti-human Hashtag 4 Human AGTAAGTTCAGCGTA
TotalSeq™-C 0255 anti-human Hashtag 5 Human AAGTATCGTTTCGCA
TotalSeq™-C 0301 anti-mouse Hashtag 1 Mouse ACCCACCAGTAAGAC
TotalSeq™-C 0302 anti-mouse Hashtag 2 Mouse GGTCGAGAGCATTCA
TotalSeq™-C 0303 anti-mouse Hashtag 3 Mouse CTTGCCGCATGTCAT
TotalSeq™-C 0304 anti-mouse Hashtag 4 Mouse AAAGCATTCTTCACG
TotalSeq™-C 0305 anti-mouse Hashtag 5 Mouse CTTTGTCTTTGTGAG

No Results Found. Please try a different search term.