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Stem Cell Lineage Tracker 2

In order to better phenotype stem cells at their various stages of development, it is important to know what markers they express (and don’t express) and having the right reagents at hand to detect them. This section gives a brief overview of common markers found on the various intermediate stages and indicates where markers may diverge between human and mouse equivalents. To see how these cells develop, view the full picture with our Stem Cell and Development Poster.

 

Embryonic Stem Cells
CD9,  CD24,  CD29,  CD49f,  CD117,  CD133,  CD324,  SSEA-1,  SSEA-3,  SSEA-4,  Tra-1-60,  Tra-1-81

Ectoderm
CD15,  CD29,  CD49f,  CD133,  GFAP,  Nestin,  Neurofilament, Olig2, Pax6,  SOX2

Endoderm CXCR4,  FOXA2,  GATA4, GATA6, SOX17
 

Mesoderm
CD10,  CD16/32,  CD34,  CD38,  CD45RA,  CD48,  CD90,  CD117,  CD123,  CD127,  CD133,  CD135,  CD150,  Sca-1
 

Hemangioblasts
Human: CD133
Mouse: Brachyury, CD135

 

Multipotent Progenitor
Human: CD34,  CD90,  CD135,  Lin-
Mouse: CD34-,  CD48-,  CD117,  CD150,  Sca-1  

 

Common Lymphoid Progenitor
(Differentiates into B cells, T cells, and ILC)
Human: CD10,  CD34,  CD45RA,  CD135,  Lin- 
Mouse: CD117low,  CD127,  CD135,  Lin-

 

Granulocyte-Monocyte Progenitor
(Differentiates into Monocytes, Macrophages, Dendritic Cells, and Granulocytes)
Human: CD34,  CD38,  CD115,  CD116,  CD123 
Mouse: CD16/32,  CD34,  CD117,  Sca-1
 

Hematopoietic Stem Cells
Human: CD34,  CD90,  CD135,  Lin-
Mouse: CD34-,  CD48-,  CD117,  CD150,  Sca-1  

 

Common Myeloid Progenitor
Human: CD34,  CD38,  CD45RA-,  CD135 
Mouse: CD34,  CD117,  CD135,  Sca-1-   

 

Megakaryocyte-Erythrocyte Progenitor
(Differentiates into RBCs and Megakaryocytes)
Human: CD34,  CD71,  CD110 
Mouse: CD34-,  CD41,  CD105,  CD150
 
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