Ultra-LEAF™ Purified anti-mouse M-CSF Recombinant Antibody

Pricing & Availability
Clone
QA19A27 (See other available formats)
Regulatory Status
RUO
Other Names
CSF1, CSF-1, MCSF
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A27_ULEAF_M-CSF_Recombinant_Antibody_040821.png
Recombinant mouse M-CSF (Cat. No. 576406) (black circles) induces the proliferation of M-NFS-60 mouse musculus cells. Ultra-LEAF™ purified anti-mouse M-CSF antibody (clone QA19A27) (purple squares) inhibits the effect of recombinant mouse M-CSF at 20 ng/mL in M-NFS-60 cells in a dose-dependent manner. The ND50 = 2 – 10 µg/mL.
  • QA19A27_ULEAF_M-CSF_Recombinant_Antibody_040821.png
    Recombinant mouse M-CSF (Cat. No. 576406) (black circles) induces the proliferation of M-NFS-60 mouse musculus cells. Ultra-LEAF™ purified anti-mouse M-CSF antibody (clone QA19A27) (purple squares) inhibits the effect of recombinant mouse M-CSF at 20 ng/mL in M-NFS-60 cells in a dose-dependent manner. The ND50 = 2 – 10 µg/mL.
Cat # Size Price Quantity Check Availability Save
935803 100 µg $245.00
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935804 1 mg $650.00
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Description

M-CSF was first characterized as a glycoprotein that induces monocyte and macrophage colony formation from precursors in murine bone marrow cultures. M-CSF binds CD14+ monocytes and promotes the survival/proliferation of peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions including phagocytic activity, microbial killing, and cytotoxicity for tumor cells as well as induces the synthesis of inflammatory cytokines such as IL-1, TNFα, and IFNγ in monocytes.

Multiple CSF1 mRNA species have been described that arise from alternative splicing in exon 6 and the alternative use of the 3’ end of exons 9 or 10. As a result, two distinct CSF1 protein products are encoded by these transcripts: a cell-surface or membrane-bound form of CSF1 (mCSF1) and a soluble form (sCSF1). Uterine sCSF1 is highly increased during pregnancy. On the contrary, uterine mCSF1 remains low during pregnancy. High levels of M-CSF have been associated to different pathologies such as pulmonary fibrosis and atherosclerosis.

M-CSF binds to its receptor M-CSFR, and this receptor is shared by a second ligand, IL-34. Mouse M-CSF and IL-34 exhibit cross-species specificity, both bind to the human and mouse M-CSF receptors. IL-34 can regulate myeloid development and substitute for CSF-1 in vivo. IL-34 has overlapping but not identical biological activities as M-CSF.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant mouse M-CSF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by the inhibition of proliferation induced by mouse recombinant M-CSF (0.02 µg/mL) on M-NFS-60 mouse musculus cells. ND50 range: 2-10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2892497 (BioLegend Cat. No. 935803)
AB_2892497 (BioLegend Cat. No. 935804)

Antigen Details

Structure
Disulfide-linked glycosylated homodimer.
Distribution

M-CSF is broadly expressed in adult mouse tissues. M-CSF is released by fibroblasts, breast cancer cell lines, alveolar macrophages, stromal bone marrow cells, endothelial cells, and mesenchymal cells.

Function
M-CSF is the key regulator of the survival, proliferation, and differentiation of mononuclear phagocytes and plays a central role in the regulation of osteoclastogenesis. CSF-1 also regulates the development of Paneth cells, Langerhans cells, lamina propria dendritic cells, and microglia.
Interaction
Monocytes, macrophages, mononuclear phagocyte precursors, microglia, proliferating smooth muscle cells, umbilical vein endothelial cells, and breast cancer cell lines.
Ligand/Receptor
M-CSFR or CSF1R (CD115)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Kawasaki ES, et al. 1985. Science. 230:291-6.
  2. Wei S, et al. 2010. J Leukocyte Biol. 88:495-505.
  3. MacDonald KP, et al. 2010. Blood. 116:3955-63.
  4. Hodge JM, et al. 2011. Plos One. 6:e21462.
  5. Morandi et al. 2011. Plos One. 6:e27450.
  6. Erblich B, et al. 2011. Plos One. 6:e26317.
Gene ID
12977 View all products for this Gene ID
UniProt
View information about M-CSF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 04/07/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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