Ultra-LEAF™ Purified anti-mouse IL-6 Recombinant Antibody

Pricing & Availability
Clone
QA19A65 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-6, Interferon-β2, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA19A65_ULEAF_IL-6_RECOM_Antibody_012921
Recombinant mouse IL-6 (Cat. No. 575706) (black circles) induces the proliferation of 7TD1 mouse cells. Ultra-LEAF™ purified anti-mouse IL-6 recombinant antibody (clone QA19A65) (purple squares) inhibits the effect of recombinant mouse IL-6 at 0.02 ng/mL in mouse 7TD1 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400193) (gray triangles) does not have the effect. The ND50 = 4 - 20 ng/mL.
  • QA19A65_ULEAF_IL-6_RECOM_Antibody_012921
    Recombinant mouse IL-6 (Cat. No. 575706) (black circles) induces the proliferation of 7TD1 mouse cells. Ultra-LEAF™ purified anti-mouse IL-6 recombinant antibody (clone QA19A65) (purple squares) inhibits the effect of recombinant mouse IL-6 at 0.02 ng/mL in mouse 7TD1 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400193) (gray triangles) does not have the effect. The ND50 = 4 - 20 ng/mL.
Cat # Size Price Quantity Check Availability Save
941403 100 µg $250
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941404 1 mg $655
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Description

IL-6 is a multifunctional cytokine that can regulate various immune and inflammatory responses. Several studies have suggested a crucial role for IL-6 in angiogenesis. The use of mice deficient in IL-6 (-/-) demonstrated a critical role for this protein in a mouse model of lung angiogenesis. IL-6 has been shown to cause proliferation and migration of systemic endothelial cells in culture (1). The classical responsiveness to IL-6 is governed by a receptor complex consisting of two membrane-bound subunits, an 80-kD cognate chain (IL-6R), and a ubiquitously expressed 130-kD β-chain receptor (gp130) which acts as the universal signal-transducing element for all IL-6 family cytokines (2). Alternatively, IL-6 regulation of leukocyte trafficking relies upon signaling via its soluble IL-6R (termed IL-6 trans-signaling) (3). IL-6 plays a major role in regulating neutrophil clearance during acute peritoneal inflammation; as a result of specific downregulation of neutrophil-attracting chemokine (CXCL1/KC) production (4). IL-6 is a key factor that reciprocally regulates Th17 and Foxp3 (+) Treg differentiation by inhibition of TGF-β induced Foxp3 and induction of RORγt, a Th17 lineage-specific transcription factor (5).

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant mouse IL-6
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant mouse IL-6 (Cat. No. 575706) at 0.02 ng/mL on mouse 7TD1 cells. ND50 range: 4 - 20 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This recombinant antibody clone is derived from clone MP5-20F3.

RRID
AB_2890858 (BioLegend Cat. No. 941403)
AB_2890858 (BioLegend Cat. No. 941404)

Antigen Details

Structure
Interleukin, neuropoietic cytokine family, potent lymphoid cell growth factor
Distribution

IL-6 is released by activated T cells, B cells, monocytes, macrophages, fibroblasts, epithelial cells, and endothelial cells.

Function
Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Interaction
T cells, B cells, hepatocytes, cholinergic neurons, myeloid cells
Ligand/Receptor
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130) and soluble IL-6R
Cell Type
B cells, Endothelial cells, Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Macrophages, Monocytes, T cells
Biology Area
Cell Biology, Immuno-Oncology, Immunology, Innate Immunity, Neuroinflammation, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. McClintock JY, Wagner EM. 1985, J Appl Physiol. 99(3):861-6.
2. Murakami M. 1993. Science 260:1808-1810.
3. Jones SA, et al. 2001. J. FASEB. 15:43-58.
4. Fielding CA, et al. 2008. J. Immunol. 181:2189-2195.
5. Sonderegger I, et al. 2008. Eur. J. Immunol. 38:1833-1838.

Gene ID
16193 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 03/26/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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