Ultra-LEAF™ Purified anti-mouse CX3CL1 (Fractalkine) Antibody

Pricing & Availability
Clone
A19080D (See other available formats)
Regulatory Status
RUO
Other Names
Small inducible cytokine subfamily D, member 1 (SCYD1), Neurotactin (NTT, NTN)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A19080D_ULEAF_CX3CL1_Fractalkine_Antibody_082621.png
Recombinant mouse CX3CL1 (Fractalkine) (Cat. No. 583506) (black circles) induces the chemotaxis of mouse Baf3-mCX3CR1 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-mouse CX3CL1 (Fractalkine) antibody (clone A19080D) (purple squares) neutralizes the chemotaxis of recombinant mouse CX3CL1 (Fractalkine) at 2 µg/mL on mouse Baf3-mCX3CR1 cells in a dose-dependent manner whereas the GoInVivo™ purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400666) (gray triangles) does not have the effect. ND50 range: 2-10 µg /mL.
  • A19080D_ULEAF_CX3CL1_Fractalkine_Antibody_082621.png
    Recombinant mouse CX3CL1 (Fractalkine) (Cat. No. 583506) (black circles) induces the chemotaxis of mouse Baf3-mCX3CR1 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-mouse CX3CL1 (Fractalkine) antibody (clone A19080D) (purple squares) neutralizes the chemotaxis of recombinant mouse CX3CL1 (Fractalkine) at 2 µg/mL on mouse Baf3-mCX3CR1 cells in a dose-dependent manner whereas the GoInVivo™ purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400666) (gray triangles) does not have the effect. ND50 range: 2-10 µg /mL.
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938903 100 µg $245.00
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938904 1 mg $650.00
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Description

CX3CL1 is a unique chemokine subclass, synthesized as a transmembrane molecule. It consists of an extracellular NH2-terminal domain, a mucin-like stalk, a transmembrane α helix, and a short cytoplasmic tail. CX3CL1 exists in two forms: as a membrane-anchored or as a shed 80-95K glycoprotein. Soluble CX3CL1 is generated by limited proteolysis on the cell surface, and a disintegrin and metallopeptidase 10 (ADAM10) and ADAM17/tumor necrosis factor-α-converting enzyme (ADAM17/TACE) participate in this shedding. It has been suggested that ADAM10 acts in the constitutive shedding, and ADAM17 acts in response to cell activation. CX3CL1 is associated with the development of different diseases such as rheumatoid arthritis (RA), rheumatoid vasculitis (RV), Sjögren’s syndrome (SS), systemic lupus erythematosus (SLE), scleroderma, multiple sclerosis, and atherosclerosis. Soluble fractalkine has been detected in the cerebrospinal fluid of patients with neuropsychiatric lupus, and is present at higher levels in the serum of patients with SS, RA, and type 2 diabetes than in control subjects.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CX3CL1 (Fractalkine)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CX3CL1 (Fractalkine) (2 µg /mL) on mouse Baf3-mCX3CR1 transfectant chemoattractant cells. ND50 range: 2-10 ug/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2894524 (BioLegend Cat. No. 938903)
AB_2894524 (BioLegend Cat. No. 938904)

Antigen Details

Structure
Chemokine
Distribution

Microglia, endothelial cells, fibroblasts, neuronal cells, hepatocytes, smooth muscle cells, monocytes, adipocytes, and human astrocytes stimulated by TNF-α and IFN-γ. CX3CL1 is also expressed in macrophages, fibroblasts, endothelial, and dendritic cells in RA synovial tissue

Function
CX3CL1 has potent chemoattractant activity for T cells, monocytes, and natural killer cells, and the expression of CX3CL1 in activated endothelial cells promotes strong adhesion of those leukocytes. CXC3L1 is induced in endothelial cells by proinflammatory cytokines (TNF-α, IFN-γ, IL-1β) and LPS.
Interaction
Monocytic cells, smooth muscle cells, T cells, B lymphocytes, NK cells, microglia, and astrocytes
Ligand/Receptor
CX3CR1
Cell Type
B cells, Endothelial cells, Microglia
Biology Area
Cell Biology, Neuroscience, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Bazan JF, et al. 1997. Nature. 385:640.
  2. Hundhausen C, et al. 2003. Blood. 102:1186.
  3. Sato E, et al. 2006. Ann Rheum Dis. 65:1257.
  4. Hundhausen C, et al. 2007 J Immunol. 178:8064.
  5. Jones B, et al. 2011. Front Immunol. 2:82.
  6. Hamann I, et al. 2011. Immunology. 133:62.
  7. Shah R, et al. 2011. Diabetes. 60:1512.
Gene ID
20312 View all products for this Gene ID
UniProt
View information about CX3CL1 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08/26/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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