Ultra-LEAF™ Purified anti-mouse CD95 (Fas) Antibody

Pricing & Availability
Clone
SA362F7 (See other available formats)
Regulatory Status
RUO
Other Names
Fas, ALPS1A, APO-1, APT1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
SA362F7_ULEAF_CD95_Antibody_111116
C57BL/6 thymocytes were stained with purified anti-mouse CD95 (clone SA362F7) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by PE anti-mouse IgG2a.
  • SA362F7_ULEAF_CD95_Antibody_111116
    C57BL/6 thymocytes were stained with purified anti-mouse CD95 (clone SA362F7) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by PE anti-mouse IgG2a.
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152803 500 µg $340.00
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152804 1 mg $625.00
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Description

CD95, also known as Fas, is an approximately 45 kD type I transmembrane protein belonging to the TNFR superfamily (TNFRSF6). The expression of CD95 has been described in various organs, such as thymus, spleen, liver, heart, lung and ovary. Upon ligand (FasL) binding, CD95 forms the death-inducing signaling complex (DISC) intracellularly and induces apoptosis of the cell. CD95-induced apoptosis plays important roles in development, as well as in maintaining peripheral tolerance of the immune system.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Fas Transfectants
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
FA - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone SA362F7 induces apoptosis when cultured together with mouse thymocytes; this can be seen with increased Annexin V and 7AAD signals.

RRID
AB_2632788 (BioLegend Cat. No. 152803)
AB_2632789 (BioLegend Cat. No. 152804)

Antigen Details

Structure
CD95 (Fas) is an approximately 45 kD type I transmembrane protein; cystiene-rich domain, death domain.
Distribution
Thymus, spleen, liver, heart, lung, and ovary.
Function
Induction of apoptosis.
Interaction
Caspase 8, caspase 10, CFLAR, FADD, PDCD6, SUMO1, and TNFRSF6.
Ligand/Receptor
FasL.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Ogasawara J, et al. 1995. J. Exp. Med. 181:485.
2. Ogasawara J, et al. 1993. Nature 364:806.
3. Nishimura Y, et al. 1995. J. Immunol. 154:4395.

Gene ID
14102 View all products for this Gene ID
UniProt
View information about CD95 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/15/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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