Ultra-LEAF™ Purified anti-mouse CD70 Antibody

Pricing & Availability
Clone
A19034C (See other available formats)
Regulatory Status
RUO
Other Names
CD27 ligand, CD70, Ki-24 antigen, TNFSF7
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A19034C_ULEAF_CD70_Antibody_1_030821
Recombinant mouse CD27L (CD70) (Cat. No. 550406) (black circles) binds to immobilized recombinant mouse CD27 (TNFRSF7)-Fc chimera (Cat. No. 765604). Ultra-LEAF™ Purified anti-mouse CD70 antibody (clone A19034C) (purple squares) inhibits the binding in a dose-dependent manner whereas the GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.14 µg/mL of recombinant mouse CD27L (CD70) to 2 µg/mL of immobilized recombinant mouse CD27 (TNFRSF7)-Fc chimera with an ND50 = 0.3 – 1.5 µg/mL.
  • A19034C_ULEAF_CD70_Antibody_1_030821
    Recombinant mouse CD27L (CD70) (Cat. No. 550406) (black circles) binds to immobilized recombinant mouse CD27 (TNFRSF7)-Fc chimera (Cat. No. 765604). Ultra-LEAF™ Purified anti-mouse CD70 antibody (clone A19034C) (purple squares) inhibits the binding in a dose-dependent manner whereas the GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.14 µg/mL of recombinant mouse CD27L (CD70) to 2 µg/mL of immobilized recombinant mouse CD27 (TNFRSF7)-Fc chimera with an ND50 = 0.3 – 1.5 µg/mL.
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160801 100 µg $155.00
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160802 1 mg $645.00
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Description

CD70, or CD27L, is a 50 kD type II transmembrane glycoprotein and member of the tumor necrosis factor superfamily. Mouse CD27L and its human counterpart share about 56% amino acid sequence identity. The interaction between CD27L and CD27, a protein of the TNF receptor superfamily expressed on NK cells and subsets of T and B cells, provides costimulatory signals that are required for T cell proliferation, clonal expansion, and promotion of effector T cell formation. CD27L-CD27 interaction on mouse B cells on the other hand, has been shown to inhibit terminal differentiation of B cells into plasma cells. CD27L expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. CD27 interaction with recombinant soluble CD27L protein (sCD27L), has shown that sCD27L enhances IFN-γ secretion by CD56 (bright) NK cells in the presence of IL-12, and these NK cells were able to suppress HCV replication. Also, CD8+ T cells stimulated with sCD27L in the presence of Ag showed enhanced proliferation and production of IL-2 and IFN-γ in vitro. In addition, administration of Ag and soluble CD27L induces expansion of Ag-specific CD8+ T cells in vivo. Data suggests that sCD27L is a potent stimulator of antiglioma immune responses that depend on CD8+ T cells; therefore, sCD27L might be an adjuvant for future immunotherapy trials for glioblastoma.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD70
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between mouse CD27 (TNFRSF7) and mouse CD27L (CD70). The ND50 range: 0.3 – 1.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application

RRID
AB_2890724 (BioLegend Cat. No. 160801)
AB_2890724 (BioLegend Cat. No. 160802)

Antigen Details

Structure
Homodimer
Distribution

Activated T cells, B cells, NK cells, antigen presenting cells, activated plasmacytoid dendritic cells (pDCs), chronic B cell lymphocytic leukemia, and large B cell lymphomas.

Function
Role in T cell proliferation, generation of cytolytic and memory T cells, B cell proliferation and differentiation. CD27L is induced by antigen receptor activation in B cells and downregulated by interaction with its receptor CD27.
Interaction
T cells, B cells, NK cells.
Ligand/Receptor
CD27
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Cancer Biomarkers, Cell Proliferation and Viability, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Bowman MR, et al. 1994. J Immunol. 152:1756-61.
  2. Shaw J, et al. 2010. Blood. 115:3051-7.
  3. Keller AM, et al. 2009. Blood. 113:5167-75.
  4. Tesselaar K, et al. 1997. J Immunol. 159:4959-65.
  5. Akiba H, et al. 1999. J Immunol. 162:7058-66.
  6. Miller J, et al. 2010. J Neurosurg. 113:280-5.
  7. Kuka M, et al. 2013. J Immunol. 191:2282-9.
  8. Jang YS, et al. 2013. Clin Immunol. 149:379-87.
Gene ID
21948 View all products for this Gene ID
UniProt
View information about CD70 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/08/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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