Ultra-LEAF™ Purified anti-mouse CD126 (IL-6Rα chain) Antibody

Pricing & Availability
Clone
D7715A7 (See other available formats)
Regulatory Status
RUO
Other Names
IL-6 Receptor α chain, IL-6R
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
D7715A7_LEAF_090407
C57BL/6 mouse splenocytes stained with LEAF™ purified D7715A7, followed by biotinylated anti-rat IgG and Sav-PE
  • D7715A7_LEAF_090407
    C57BL/6 mouse splenocytes stained with LEAF™ purified D7715A7, followed by biotinylated anti-rat IgG and Sav-PE
Cat # Size Price Quantity Avail. Save
115815 100 µg $165.00
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115816 1 mg $610.00
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Description

CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation. The D7715A7 (15A7) antibody blocks IL-6/IL-6 receptor interactions.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
OKT4 hybridoma cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block, IP, Neut - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,5in vivo receptor blocking1-2,4-6,8, and in vitro neutralization7. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115804) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115808).

Please note, this clone had previously reported reactivity to rat, however upon further testing it has been determined to not react with rat.

Application References

(PubMed link indicates BioLegend citation)
  1. Coulie PG, et al. 1990. Curr. Top. Microbiol. Immunol. 166:43. (Block, IP)
  2. Vink A, et al. 1990. J. Exp. Med. 172:997. (Block, IP)
  3. Sun R, et al. 2004. J. Immunol. 172:5648. (FC)
  4. McKinney WJ, et al. 1998. Am. J. Respir. Cell Mol. Biol. 18:696. (Block)
  5. Weigert C, et al. 2006. J. Biol. Chem. 281:7060. (Block, IP)
  6. Koizumi N, et al. 2007. J. Immunol. 178:1767. (Block)
  7. Walker F, et al. 2008. Blood 111:3978. (Neut) PubMed
  8. Yadav M, et al. 2008. J. Immunol. 180:2772. (Block) PubMed
  9. Sintes J, et al. 2010. J. Leukoc. Biol. 88:687. PubMed
RRID
AB_2810349 (BioLegend Cat. No. 115815)
AB_2810350 (BioLegend Cat. No. 115816)

Antigen Details

Structure
Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
Distribution

Activated T cells and B cells, monocytes, hepatocytes, plasma cells

Function
Stimulates T cells, B cells, hematopoietic precursor proliferation and differentiation
Ligand/Receptor
IL-6
Cell Type
B cells, Mesenchymal Stem Cells, Monocytes, Plasma cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taga T, et al. 1997. Annu. Rev. Immunol. 15:797.
2. Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London.
3. Boulanger MJ, et al. 2003. Science 300:2101.

Gene ID
16194 View all products for this Gene ID
UniProt
View information about CD126 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 04/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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