- GoH3 (See other available formats)
- Regulatory Status
- HCDM listed
- Other Names
- VLA-6 α chain, α6 integrin, integrin α6, ITGA6
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, and epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.Product Details
- Human, African Green, Mouse, Baboon, Capuchin Monkey, Cat (Feline), Cattle (Bovine, Cow), Chimpanzee, Cynomolgus, Dog (Canine), Horse (Equine), Rabbit (Lapine), Rhesus, Sheep (Ovine), Swine (Pig, Porcine)
- Antibody Type
- Host Species
- Mouse mammary tumor cells
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
ICC - Verified
IHC-F, IP, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin a61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin a6 function in vivo.
(PubMed link indicates BioLegend citation)
- Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
- Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
- Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
- Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
- Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
- Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
- Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
- Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
- Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
- Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
- Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
- Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
- Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
AB_2814165 (BioLegend Cat. No. 313637)
AB_2814166 (BioLegend Cat. No. 313638)
- Integrin family, associates with β1 or β4, 120 kD
Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells
- Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
- With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
- Cell Type
- Embryonic Stem Cells, Endothelial cells, Epithelial cells, Monocytes, Platelets, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.
- Gene ID
- 16403 View all products for this Gene ID 3655 View all products for this Gene ID
- View information about CD49f on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
|Purified anti-human/mouse CD49f||GoH3||FC, ICC, IHC-F, IP, Block|
|Biotin anti-human/mouse CD49f||GoH3||FC|
|FITC anti-human/mouse CD49f||GoH3||FC|
|Alexa Fluor® 488 anti-human/mouse CD49f||GoH3||FC|
|Alexa Fluor® 647 anti-human/mouse CD49f||GoH3||FC|
|PE anti-human/mouse CD49f||GoH3||FC|
|APC anti-human/mouse CD49f||GoH3||FC|
|PerCP/Cyanine5.5 anti-human/mouse CD49f||GoH3||FC|
|Pacific Blue™ anti-human/mouse CD49f||GoH3||FC|
|PE/Cyanine7 anti-human/mouse CD49f||GoH3||FC|
|Brilliant Violet 421™ anti-human/mouse CD49f||GoH3||FC, IHC-P|
|PE/Dazzle™ 594 anti-human/mouse CD49f||GoH3||FC|
|APC/Cyanine7 anti-human/mouse CD49f||GoH3||FC|
|APC/Fire™ 750 anti-human/mouse CD49f||GoH3||FC|
|TotalSeq™-A0070 anti-human/mouse CD49f||GoH3||PG|
|TotalSeq™-C0070 anti-human/mouse CD49f||GoH3||PG|
|Ultra-LEAF™ Purified anti-human/mouse CD49f||GoH3||FC, ICC, IHC-F, IP, Block|
|TotalSeq™-B0070 anti-human/mouse CD49f||GoH3||PG|
|TotalSeq™-D0070 anti-human/mouse CD49f||GoH3||PG|
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human/mouse CD49f
Biotin anti-human/mouse CD49f
FITC anti-human/mouse CD49f
Alexa Fluor® 488 anti-human/mouse CD49f
Alexa Fluor® 647 anti-human/mouse CD49f
PE anti-human/mouse CD49f
APC anti-human/mouse CD49f
PerCP/Cyanine5.5 anti-human/mouse CD49f
Pacific Blue™ anti-human/mouse CD49f
PE/Cyanine7 anti-human/mouse CD49f
Brilliant Violet 421™ anti-human/mouse CD49f
PE/Dazzle™ 594 anti-human/mouse CD49f
APC/Cyanine7 anti-human/mouse CD49f
APC/Fire™ 750 anti-human/mouse CD49f
TotalSeq™-A0070 anti-human/mouse CD49f
TotalSeq™-C0070 anti-human/mouse CD49f
Ultra-LEAF™ Purified anti-human/mouse CD49f
TotalSeq™-B0070 anti-human/mouse CD49f
TotalSeq™-D0070 anti-human/mouse CD49f