- H5C6 (See other available formats)
- Regulatory Status
- HCDM listed
- Other Names
- LIMP, LAMP-3, Melanoma-associated antigen (ME491), Pltgp40, gp55
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6.Product Details
- Human, African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- T cell line HPB-ALL
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
IHC-P - Verified
ICC, IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence2, and immunoprecipitation1.
(PubMed link indicates BioLegend citation)
- Hildreth JE, et al. 1991. Blood 77:121. (IP, WB)
- Beatty WL, et al. 2006. J. Cell Sci. 119:350. (IF)
- Product Citations
AB_2800940 (BioLegend Cat. No. 353039)
AB_2800941 (BioLegend Cat. No. 353040)
- Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle
- Platelet activation
- CD9, CD81, VLA-3, and VLA-6
- Cell Type
- B cells, Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
- Azorsa DO, et al. 1991. Blood 78:280.
- Kishimoto T, et al. Eds. 1997. Leukocyte Typing V1. Oxford University Press New York.
- Hildreth JE, et al. 1991. Blood 77:121.
- Anzai N, et al. 2002. Blood 99:4413.
- Gene ID
- 967 View all products for this Gene ID
- View information about CD63 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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