- TL2.1 (See other available formats)
- Regulatory Status
- HCDM listed
- Other Names
- Toll like receptor 2, TIL4, CD282
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products.Product Details
- Antibody Type
- Host Species
- Human TLR2-transfected CHO cells
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
Block, IHC-P, IHC-F, IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation. Additional reported applications (for the relevant formats) include: inhibition of PGP activity and blocking of cytokine production1,3,7, immunoprecipitation1, immunohistochemistry of 4% paraformaldehyde-fixed frozen sections2 and immunohistochemistry of HOPE-fixed (HEPES-glutamic acid buffer-mediated organic solvent protection effect) paraffin-embedded sections4 , and Western blotting2. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 309716) (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered).
(PubMed link indicates BioLegend citation)
- Flo T, et al. 2000. J. Immunol. 164:2064.
- Faure E, et al. 2001. J. Immunol. 166:2018.
- Sugawara S, et al. 2001. Infect. Immun. 69:4951.
- Droemann D, et al. 2003. Histochem. Cell Biol. 119:103.
- Chavakis E, et al. 2007. Circ. Res. 100:204.PubMed
- Fiala M, et al. 2007. Proc. Natl. Acad. Sci. USA 10.1073/P. Natl. Acad. Sci. USA.0701267104.
- Goo SY, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200.PubMed
- Weiss DJ,et al. 2008.J. Leukoc. Biol. 83:48. PubMed
- Harris, KM., et al. 2011. J. Leukoc Biol. 90:727. PubMed.
- Elass-Rocahrd E, et al. 2012. J Biol Chem. 287:34432. PubMed.
AB_2810467 (BioLegend Cat. No. 309717)
AB_2810468 (BioLegend Cat. No. 309718)
- IL-1R superfamily, TLR family, type 1 transmembrane protein, 90 kD
Monocytes, granulocytes, endothelial and epithelial cells
- Activates innate host defense against bacterial pathogens
- Microbial lipoproteins, peptidoglycans, lipoteichoic acid
- Cell Type
- Endothelial cells, Epithelial cells, Granulocytes, Monocytes, Neutrophils, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Toll Like Receptors
- Antigen References
1. Lien E, et al. 1999. J. Biol. Chem. 274:33419.
2. Lien E, et al. 2001. J. Biol. Chem. 276:1873.
3. Sabroe I, et al. 2002. J. Immunol. 168:4701
- Gene ID
- 7097 View all products for this Gene ID
- View information about CD282 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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