- A15083D (See other available formats)
- Regulatory Status
- Other Names
- Macrophage derived cytokine (MDC), Small inducible cytokine subfamily A, member 22 (Scya22), stimulated T cell chemotactic protein (STCP-1), ABCD-1, DC/B-CK, A-152E5.1
- Mouse IgG2a, κ
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- Product Citations
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CCL22 was initially cloned from human activated macrophages by random sequencing of cDNA clones. CCL22 was described originally as a constitutively produced, thymus-specific chemokine engaged in the recruitment of T cells. CCL22 and CCL17 bind to CCR4, and both chemokines share similar in vivo expression patterns and are closely linked on human chromosome 16; nevertheless, they are only 37% identical at the amino acid level. CCL22 has been associated to different diseases such as allergen-induced lung inflammation, atopic dermatitis, and lymphoma. Also, high levels of CCL22 have been detected in the CNS in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). In addition, CCR4+ T cells have been linked to endotoxic shock, rheumatoid arthritis, T cell lymphoma, and autoimmune diabetes. Recruitment of Tregs to human cancers producing CCL22 suppresses tumor-specific T cell immunity and contributes to tumor growth. In the context of central nervous system, CCR4 is expressed in astrocytes, microglia, and in hippocampal neurons. Most recently, CCR4 and CCL22 have been detected in the anterior hypothalamus/preoptic area, a key region involved in thermogenesis. In this sense, it has been described that CCL22 is a prostaglandin-dependent pyrogen, acting in the anterior hypothalamus to induce hyperthermia via activation of brown adipose tissue.Product Details
- Antibody Type
- Host Species
- Recombinant human CCL22
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by neutralizing activity. The ND50 is 1.5 - 4.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
AB_2650729 (BioLegend Cat. No. 694403)
AB_2650730 (BioLegend Cat. No. 694404)
Macrophages, NK cells, IFNγ stimulated human keratinocytes, thymic epithelial cells and intestinal epithelial cells.
- Induces the migration of CCR4+ Th2 cells, Tregs, DC, and NK cells. Induced by LPS and CD40 antibody in macrophages. Upregulated by Th2 cytokines (IL-4 and IL-5) and downregulated by Th1 cytokines (IFNγ).
- Dendritic cells, NK cells, Th2 cells, T regs, CLA+ skin-homing memory/effector T cells.
- Cell Type
- Epithelial cells, Macrophages, NK cells
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- Antigen References
1. Godiska R, et al. 1997. J. Exp. Med. 185:1595.
2. Imai T, et al. 1998. J. Biol. Chem. 273(3):1764-8.
3. Yamashita U and Kuroda E. 2002. Crit. Rev. Immunol. 22:105.
4. Horikawa T, et al. 2002. Int. Immunol. 14:767.
5. Mailloux AW, et al. 2009. J. Immunol. 182:2753.
6. Dogan RN, et al. 2011. J. Leukoc. Biol. 89:93.
7. Osborn O, et al. 2011. Cytokine 53:311.
- Gene ID
- 6367 View all products for this Gene ID
- View information about CCL22 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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