Ultra-LEAF™ Purified anti-human CCL22 Antibody

Pricing & Availability
Clone
A15083D (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage derived cytokine (MDC), Small inducible cytokine subfamily A, member 22 (Scya22), stimulated T cell chemotactic protein (STCP-1), ABCD-1, DC/B-CK, A-152E5.1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A15083D_ULEAF_CCL22_NEUT_Antibody_020717
Recombinant human CCL22 chemoattracts Baf3-hCCR4 transfectants in a dose dependent manner (black squares). Anti-human CCL22 monoclonal antibody (purple circles) neutralizes the chemoattraction of Baf3-hCCR4 transfectants induced by recombinant human CCL22 (1.2 ng/ml). The ND50 = 0.4 - 2.4 µg/ml.
  • A15083D_ULEAF_CCL22_NEUT_Antibody_020717
    Recombinant human CCL22 chemoattracts Baf3-hCCR4 transfectants in a dose dependent manner (black squares). Anti-human CCL22 monoclonal antibody (purple circles) neutralizes the chemoattraction of Baf3-hCCR4 transfectants induced by recombinant human CCL22 (1.2 ng/ml). The ND50 = 0.4 - 2.4 µg/ml.
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694403 100 µg $245.00
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694404 1 mg $650.00
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Description

CCL22 was initially cloned from human activated macrophages by random sequencing of cDNA clones. CCL22 was described originally as a constitutively produced, thymus-specific chemokine engaged in the recruitment of T cells. CCL22 and CCL17 bind to CCR4, and both chemokines share similar in vivo expression patterns and are closely linked on human chromosome 16; nevertheless, they are only 37% identical at the amino acid level. CCL22 has been associated to different diseases such as allergen-induced lung inflammation, atopic dermatitis, and lymphoma. Also, high levels of CCL22 have been detected in the CNS in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). In addition, CCR4+ T cells have been linked to endotoxic shock, rheumatoid arthritis, T cell lymphoma, and autoimmune diabetes. Recruitment of Tregs to human cancers producing CCL22 suppresses tumor-specific T cell immunity and contributes to tumor growth. In the context of central nervous system, CCR4 is expressed in astrocytes, microglia, and in hippocampal neurons. Most recently, CCR4 and CCL22 have been detected in the anterior hypothalamus/preoptic area, a key region involved in thermogenesis. In this sense, it has been described that CCL22 is a prostaglandin-dependent pyrogen, acting in the anterior hypothalamus to induce hyperthermia via activation of brown adipose tissue.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CCL22
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralizing activity. The ND50 is 1.5 - 4.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2650729 (BioLegend Cat. No. 694403)
AB_2650730 (BioLegend Cat. No. 694404)

Antigen Details

Structure
Chemokine
Distribution

Macrophages, NK cells, IFNγ stimulated human keratinocytes, thymic epithelial cells and intestinal epithelial cells.

Function
Induces the migration of CCR4+ Th2 cells, Tregs, DC, and NK cells. Induced by LPS and CD40 antibody in macrophages. Upregulated by Th2 cytokines (IL-4 and IL-5) and downregulated by Th1 cytokines (IFNγ).
Interaction
Dendritic cells, NK cells, Th2 cells, T regs, CLA+ skin-homing memory/effector T cells.
Ligand/Receptor
CCR4
Cell Type
Epithelial cells, Macrophages, NK cells
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Godiska R, et al. 1997. J. Exp. Med. 185:1595.
2. Imai T, et al. 1998. J. Biol. Chem. 273(3):1764-8.
3. Yamashita U and Kuroda E. 2002. Crit. Rev. Immunol. 22:105.
4. Horikawa T, et al. 2002. Int. Immunol. 14:767.
5. Mailloux AW, et al. 2009. J. Immunol. 182:2753.
6. Dogan RN, et al. 2011. J. Leukoc. Biol. 89:93.
7. Osborn O, et al. 2011. Cytokine 53:311. 

Gene ID
6367 View all products for this Gene ID
UniProt
View information about CCL22 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 04/27/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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