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Ultra-LEAF™ Purified anti-human BMP-7 Antibody

Pricing & Availability
Clone
A17135E (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17135E_ULEAF_BMP7_Antibody_090821.png
Recombinant human BMP-7 (Cat. No. 595602) (black circles) induces the differentiation of ATDC5 mouse chondrogenic cells. Ultra-LEAF™ purified anti-human BMP-7 antibody (clone A17135E) (purple squares) inhibits the effect of recombinant human BMP-7 at 0.2 µg /mL on ATDC5 cells in a dose- dependent manner, whereas the GoInVivo™ purified mouse IgG2b, κ isotype ctrl antibody (gray triangles) (Cat. No. 400366) does not have the effect. ND50 range: 0.3 - 1.5 μg/mL.
  • A17135E_ULEAF_BMP7_Antibody_090821.png
    Recombinant human BMP-7 (Cat. No. 595602) (black circles) induces the differentiation of ATDC5 mouse chondrogenic cells. Ultra-LEAF™ purified anti-human BMP-7 antibody (clone A17135E) (purple squares) inhibits the effect of recombinant human BMP-7 at 0.2 µg /mL on ATDC5 cells in a dose- dependent manner, whereas the GoInVivo™ purified mouse IgG2b, κ isotype ctrl antibody (gray triangles) (Cat. No. 400366) does not have the effect. ND50 range: 0.3 - 1.5 μg/mL.
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946903 100 µg $358
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946904 1 mg $1205
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Description

Bone morphogenetic proteins (BMPs) belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, and are composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. BMP-7, originally identified by its properties to induce bone formation, has been identified as an anti-fibrotic molecule, antagonizing TGF-β1. Fibrosis is associated with the emergence of fibroblasts derived from endothelial cells, suggesting an endothelial-mesenchymal transition (EndMT). TGF-β1 induces endothelial cells to undergo EndMT, whereas bone BMP-7 preserved the endothelial phenotype. BMP-7 inhibits fibrosis in the kidney, lung, liver, heart, peritonium, oral submucosa tissue, and colonic wall.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human BMP-7
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of differentiation induced by recombinant human BMP-7 (Cat. No. 595602) at 0.2 µg/mL on ATDC5 mouse chondrogenic cells. ND50 range: 0.3-1.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2894547 (BioLegend Cat. No. 946903)
AB_2894547 (BioLegend Cat. No. 946904)

Antigen Details

Structure
Growth factor, homodimer
Distribution

Widely expressed

Function
BMP-7 promotes bone formation; BMP-7 induces Langerhans cell differentiation and proliferation, induces brown adipocyte differentiation and thermogenesis, and it has antifibrotic properties.
Interaction
Osteoblasts, Langerhans cells, adipocytes, endothelial cells
Ligand/Receptor
BMP type-I receptor ALK3 and Type II BMP receptor
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Cytokine/Chemokine Receptors, Growth Factors, Tumor Suppressors
Antigen References
  1. Chen D, et al. 2004. Growth Factors 22:233.
  2. Tseng Y, et al. 2008. Nature 454:1000. 
  3. Zeisberg E, et al. 2007. Nat. Med. 13:952. 
  4. Hao ZM, et al. 2012. Mol. Ther. 20:2043. 
  5. Yasmin N, et al. 2013. J. Exp. Med. 210:2597.
Gene ID
655 View all products for this Gene ID
UniProt
View information about BMP-7 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/08/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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