Spark YG™ 570 anti-Synapsin I/II/III Antibody

Pricing & Availability
Clone
A17080A (See other available formats)
Regulatory Status
RUO
Other Names
SYN, Synapsin, Synapsin I, Synapsin II, Synapsin III
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17080A_SparkYG570_Synapsin_I-II-III_Antibody_1_092420.png
IHC staining of Spark YG™ 570 anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • A17080A_SparkYG570_Synapsin_I-II-III_Antibody_1_092420.png
    IHC staining of Spark YG™ 570 anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • A17080A_SparkYG570_Synapsin_I-II-III_Antibody_2_092420.png
    IHC staining of Spark YG™ 570 anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
See Spark YG™ 570 spectral data
Cat # Size Price Quantity Avail. Save
853713 25 µg $145.00
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853714 100 µg $300.00
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Description

Synapsins are a family of proteins involved in neurotransmitter release at the chemical synapses. Synapsins anchor synaptic vesicles to the cytoskeletal structures at the presynaptic terminal. Upon receiving an action potential, phosphorylation of synapsins, possibly by PKA, leads to the release of synaptic vesicles from cytoskeletal structures. This allows membrane docking and fusion of synaptic vesicles with the presynaptic membrane and neurotransmitter release. 

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein corresponding to amino acids 362-450 of human Synapsin I
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 2.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.

Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2888739 (BioLegend Cat. No. 853713)
AB_2888739 (BioLegend Cat. No. 853714)

Antigen Details

Structure
Synapsin I is a 704 amino acid protein with an apparent molecular mass of ~74 kD. Synapsin II is a 582 amino acid protein with an apparent molecular mass of ~63 kD. Synapsin III is a 580 amino acid protein with an apparent molecular mass of ~63 kD.
Distribution

Tissue distribution: Mainly in the central nervous system. Also expressed in testis.
Cellular distribution: Cytosol, nucleus, and plasma membrane.

Function
Synapsins are involved in the regulation of synaptic vesicle fusion and neurotransmitter release at the presynaptic terminals.
Interaction
CAPON (Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein), PKA
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Greengard P, et al. 1993. Science. 259(5096):780
  2. Milovanovic D, et al. 2017. Neuron. 93(5):995
  3. Evergren E, et al. 2007. J. Neurosci. Res. 85(12):2648
Gene ID
6853 View all products for this Gene ID 6854 View all products for this Gene ID 8224 View all products for this Gene ID
UniProt
View information about Synapsin I/II/III on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/24/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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