Recombinant Mouse TIM-1 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
TIM1, KIM-1, HAVCR1, CD365, Hepatitis A Virus Cellular Receptor 1, Kidney Injury Molecule 1, T-Cell Membrane Protein 1
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Recombinant mouse TIM-1 inhibits the proliferation of anti CD3-activated T cells. The ED50 for this effect is 0.15 – 0.75 µg/mL.
  • Mouse_TIM-1_CF_RECOM_1_071921.png
    Recombinant mouse TIM-1 inhibits the proliferation of anti CD3-activated T cells. The ED50 for this effect is 0.15 – 0.75 µg/mL.
  • Mouse_TIM-1_CF_RECOM_2_071921.png
    Stability Testing for Recombinant Mouse TIM-1. Recombinant mouse TIM-1 was aliquoted in PBS, pH 7.6 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4⁰C (Control). The samples were tested for their ability to inhibit the proliferation of human CD3-activated T cells. The ED50 for this effect is 0.15 – 0.75 µg/mL.
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794804 25 µg $85.00
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794806 100 µg $285.00
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Description

The TIM (T-cell/transmembrane, mucin and immunoglobulin domain) proteins are type I membrane-attached proteins. Three TIM members have been described in humans (TIM-1, TIM-3, and TIM-4) and four in mouse (TIM-1 to TIM-4). The family possesses four domains, the N-terminal Ig variable (IgV) domain, the glycosylated mucin domain, the transmembrane, and the intracellular domain. The TIM proteins bind phosphatidylserine that is exposed on the membrane of apoptotic cells. The binding of phosphatidylserine is through a pocket present in the IgV domain, a metal-ion coordination site. Mouse TIM-2 does not bind phosphatidylserine since it lacks the binding pocket. TIM-1 binds to P-selectin (CD62P) and mediates T-cell trafficking and acts as a costimulatory molecule. Human and mouse TIM-1 are able to bind to selectins in cell-free assays, with higher affinity to P-selectin than E-selectin and L-selectin. A contact hypersensitivity model showed that the interaction between P-selectin and TIM-1 plays a role in the migration of activated T cells in the inflamed skin. TIM-1 was initially identified as the hepatitis A virus cellular receptor 1, and it enhances virus infection of epithelial cells through the interaction of the TIM-1 IgV domain and the phosphatidylserine present on the viral envelope. Thus, TIM-1 interacts with hepatitis A, Hepatitis C, Ebola, dengue, Zika, and Japanese encephalitis viruses.

Product Details
Technical data sheet

Product Details

Source
Mouse TIM-1, amino acid (Tyr22-Thr212) (Accession: # Q5QNS5), with a C-terminal 6His tag, was expressed in 293E cells.
Molecular Mass
The 223 amino acid recombinant protein has a predicted molecular mass of approximately 24.4 kD. The DTT-reduced and non-reduced protein migrates at approximately 70 and 80 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Ser.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS pH7.6.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2ᵒC and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Recombinant mouse TIM-1 inhibits the proliferation of anti CD3-activated T cells. The ED50 for this effect is 0.15 – 0.75 µg/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

 

Antigen Details

Structure
Monomer
Distribution

T and B subsets, regulatory B cells, natural killer T cells, mast cells, and tubular epithelial cells after kidney injury

Function
Mediates T-cell trafficking during inflammation, acts as a costimulatory molecule, mediates the entry of hepatitis A virus to endothelial cells
Interaction
Apoptotic cells, endothelial cells, hepatitis A virus, P-selectin (CD62P), E-selectin (CD62E), L-selectin (CD62E), TIM-1
Ligand/Receptor
TIM-4, phosphatidylserine
Bioactivity
Recombinant mouse TIM-1 inhibits the proliferation of anti CD3-activated T cells.
Antigen References
  1. Austrup F, et al. 1998. Nature. 385:81.
  2. Umetsu SE, et al. 2005, Nat Immunol. 6:447. 
  3. Kobayashi N, et al. 2007. Immunity. 27:927.
  4. Santiago C, et al. 2007. Immunity. 27:941.
  5. Moller-Tank S, et al. 2013. J Virol. 87:8327.
  6. Angiari S, et al. 2014. Immunity. 40:542.
  7. Dejarnac O, et al. 2018. Cell Rep. 23:1779.
  8. Brouillette RB, et al. 2018. J Virol. 92.pii:e00093-18.
  9. Brunton B, et al. 2019. PLoS Negl Trop Dis. 13:e0006983.
Gene ID
171283 View all products for this Gene ID
UniProt
View information about TIM-1 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 1    Revision Date: 07/19/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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