Recombinant Mouse IL-9 (ELISA Std.)

Pricing & Availability
Regulatory Status
Other Names
P40, MEA (mast cell growth enhancing activity), MCGF (Mast cell growth factor), TCGF-3 (T cell growth factor-3),
Ave. Rating
Submit a Review
Product Citations
  • Human_IL-9_RECOM_ELISA_032417
Cat # Size Price Quantity Avail. Save
566901 4 pack $80.00
Check Availability

Need larger quantities of this item?
Request Bulk Quote

IL-9 was initially identified in mice as a T cell growth factor. Human and murine IL-9 show 56% amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15 and IL-21. IL-9 is expressed by activated Th9, Th17, Treg, and mast cells and appears to play important roles in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immune suppressive mast cells to sites of inflammation. Genetic studies in a mouse model of asthma demonstrated that this cytokine is a key factor in the pathogenesis of bronchial hyperresponsiveness. Indeed, it has been reported that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability and plays a role in the development of food allergies. Also, IL-9 has been associated with atopic dermatitis.

Product Details
Technical data sheet

Product Details

Mouse IL-9, (amino acids Gln19-Pro144, Accession# NP_032399), was expressed in insect cells.
Molecular Mass
The 125 amino acid recombinant protein has a predicted molecular mass of approximately 14 kD. The DTT-reduced protein migrates as multiple bands between 17-25 kD by SDS-PAGE due to differential glycosylation. The predicted N-terminal amino acid is Gln.
>95%, as determined by Coomassie stained SDS-PAGE
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Lot-specific (please contact technical support for mass/vial, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, immediately store unopened vials between 2°C and 8°C and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 250 pg/mL to 3.91 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance.

Application Notes

This IL-9 protein is useful as a standard for a mouse IL-9 sandwich ELISA, using unlabeled RM9A4 antibody as capture and biotinylated Poly5317 antibody as detection.

Antigen Details


Th2 cells, mast cells, activated Th9, Th17, and Treg cells.

Autocrine growth and activation factor for Th17, Tregs, and mast cells. IL-1, histamine, and LPS induce the expression of IL-9 in mast cells. IL-9 is induced synergistically by IL-2, IL-4, and TGFβ and inhibited by IFNγ in naïve CD4 T cells.
IL-9R complex (IL-9Rα and γ chain)
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Antigen References

1. Goswami R. and Kaplan MH. 2011. J. Immunol. 186:3283.
2. Kaiko GE. and Foster PS. 2011. Curr. Opin. Allergy Clin. Immunol. 11:39.
3. Townsend JM. et al. 2000. Immunity 13:573.
4. Mock BA. et al. 1990. Immunogenetics. 31:265.
5. Tan C. et al. 2010. J. Immunol. 185:6795.
6. Jager A. et al. 2009. J. Immunol. 183:7169.
7. Sismanopoulos N, et al. 2012. PLoS One 7(3):e33271.

Gene ID
16198 View all products for this Gene ID
View information about IL-9 on

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03/28/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Insert Note Here
Save Close Clear
Lab Timer
Remember me
Forgot your password? Reset Password
Request an Account