- Regulatory Status
- Other Names
- P40, MEA (mast cell growth enhancing activity), MCGF (Mast cell growth factor), TCGF-3 (T cell growth factor-3),
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- Product Citations
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IL-9 was initially identified in mice as a T cell growth factor. Human and murine IL-9 show 56% amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15 and IL-21. IL-9 is expressed by activated Th9, Th17, Treg, and mast cells and appears to play important roles in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immune suppressive mast cells to sites of inflammation. Genetic studies in a mouse model of asthma demonstrated that this cytokine is a key factor in the pathogenesis of bronchial hyperresponsiveness. Indeed, it has been reported that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability and plays a role in the development of food allergies. Also, IL-9 has been associated with atopic dermatitis.Product Details
- Mouse IL-9, (amino acids Gln19-Pro144, Accession# NP_032399), was expressed in insect cells.
- Molecular Mass
- The 125 amino acid recombinant protein has a predicted molecular mass of approximately 14 kD. The DTT-reduced protein migrates as multiple bands between 17-25 kD by SDS-PAGE due to differential glycosylation. The predicted N-terminal amino acid is Gln.
- >95%, as determined by Coomassie stained SDS-PAGE
- Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
- Lot-specific (please contact technical support for mass/vial, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Upon receipt, immediately store unopened vials between 2°C and 8°C and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
ELISA - Quality tested
- Recommended Usage
Each lot of this protein is quality control tested by ELISA. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 250 pg/mL to 3.91 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance.
- Application Notes
This IL-9 protein is useful as a standard for a mouse IL-9 sandwich ELISA, using unlabeled RM9A4 antibody as capture and biotinylated Poly5317 antibody as detection.
Th2 cells, mast cells, activated Th9, Th17, and Treg cells.
- Autocrine growth and activation factor for Th17, Tregs, and mast cells. IL-1, histamine, and LPS induce the expression of IL-9 in mast cells. IL-9 is induced synergistically by IL-2, IL-4, and TGFβ and inhibited by IFNγ in naïve CD4 T cells.
- IL-9R complex (IL-9Rα and γ chain)
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Antigen References
1. Goswami R. and Kaplan MH. 2011. J. Immunol. 186:3283.
2. Kaiko GE. and Foster PS. 2011. Curr. Opin. Allergy Clin. Immunol. 11:39.
3. Townsend JM. et al. 2000. Immunity 13:573.
4. Mock BA. et al. 1990. Immunogenetics. 31:265.
5. Tan C. et al. 2010. J. Immunol. 185:6795.
6. Jager A. et al. 2009. J. Immunol. 183:7169.
7. Sismanopoulos N, et al. 2012. PLoS One 7(3):e33271.
- Gene ID
- 16198 View all products for this Gene ID
- View information about IL-9 on UniProt.org