Recombinant Mouse IL-6 (ELISA Std.)

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin-6, Interferon-β2, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
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Product Citations
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575709 4 pack $80.00
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Description

IL-6 is a multifunctional cytokine that can regulate various immune and inflammatory responses. Several studies have suggested a crucial role for IL-6 in angiogenesis. The use of mice deficient in IL-6 (–/–) demonstrated a critical role for this protein in a mouse model of lung angiogenesis. IL-6 has been shown to cause proliferation and migration of systemic endothelial cells in culture. The classical responsiveness to IL-6 is governed by a receptor complex consisting of two membrane-bound subunits, an 80-kD cognate -chain (IL-6R ), and a ubiquitously expressed 130-kD β-chain receptor (gp130) which acts as the universal signal-transducing element for all IL-6 family cytokines. Alternatively, IL-6 regulation of leukocyte trafficking relies upon signaling via its soluble IL-6R (termed IL-6 trans-signaling). IL-6 plays a major role in regulating neutrophil clearance during acute peritoneal inflammation; as a result of specific down-regulation of neutrophil-attracting chemokine (CXCL1/KC) production. IL-6 is a key factor that reciprocally regulates Th17 and Foxp3(+) Treg differentiation by inhibition of TGF-beta induced Foxp3 and induction of RORgammat, a Th17 lineage-specific transcription factor.

Product Details
Technical data sheet

Product Details

Source
Mouse IL-6, amino acids Phe25-Thr211 (Accession# MUSIL6), was expressed in E. coli.
Molecular Mass
The 188 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 21,866 Da. The DTT-reduced protein migrates at approximately 22kDa and the non-reduced protein migrates at approximately 21.5kDa by SDS-PAGE.
Purity
Purity is >98% as determined by SDS PAGE, Coomassie stained with Invitrogen SimplyBlue SafeStain.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration
Lot-specific (please contact technical support for mass/vial, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IL-6 protein is useful as a standard for a mouse IL-6 sandwich ELISA, using unlabeled MP5-20F3 antibody (Cat. No. 504502) for capture and biotinylated MP5-32C11 antibody (Cat. No. 504602) for detection.

Product Citations
  1. Li H, et al. 2019. Investig Clin Urol. 60:359. PubMed

Antigen Details

Structure
Cytokine
Distribution

IL-6 is released by activated T cells, B cells, monocytes, macrophages, fibroblasts, epithelial cells, and endothelial cells.

Function
Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Interaction
T cells, B cells, hepatocytes, cholinergic neurons
Ligand/Receptor
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130) and soluble IL-6R
Bioactivity
Hematopoiesis, antigen-specific immune responses, inflammatory reactions, acute phase reactions
Biology Area
Cell Biology, Stem Cells, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. McClintock JE and Wagner EM J Appl Physiol 99:861-866 2005.
2. Murakami M Science 260:1808-1810 1993.
3. Jones SA, et al. FASEB J. 15:43-58 2001.
4. Fielding CA, et al. J. Immunol. 181:2189-2195. 2008.
5. Sonderegger I, et al. Eur. J. Immunol. 38:1833-1838 2008.

Gene ID
16193 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/29/2016

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