RAPID MAX™ Human IL-6 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
B-cell stimulatory factor 2, Interferon beta-2, Hybrid¬oma growth factor, CTL differentiation factor, B-cell differentiation factor, BSF2, BSF-2, CDF, HGF, HSF, IFNB2, IFN-beta-2, IL6, IL-6, interleukin 6, interleukin BSF-2, interleukin-6, MGI-2A
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RAPID-MAX_Human_IL-6_Kit_1_111220
  • RAPID-MAX_Human_IL-6_Kit_1_111220
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430519 1 Pre-coated Plate $465.00
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Description

Human IL-6 (also known as B-cell stimulatory factor 2, Interferon beta-2, Hy¬bridoma growth factor, and CTL differentiation factor) plays an essential role in the final differentiation of B-cells into Ig-secreting cells. It induces myeloma and plasmacytoma growth, nerve cells differentiation, and acute phase reac¬tants in hepatocytes. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. It has been shown that IL-6 plays a critical role in many physiological and pathological conditions, including autoimmune diseases, such as rheumatoid arthritis.

The BioLegend RAPID MAX™ Human IL-6 ELISA Kit is a one step, one wash, 80 minute Enzyme-Linked Immunosorbent Sandwich Assay (ELISA) with a pre-coated, 96-well strip plate that binds the biotinylated capture antibody. Standards and samples are added into the wells, followed by an antibody cocktail. The antibody cocktail consists of an biotinylated rat monoclonal capture antibody and an HRP-conjugated rat monoclonal detection antibody. This kit is specifically designed for the accurate quantitation of human IL-6 from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • RAPID MAX™ Pre-coated 96-well Strip Microplate
  • Human IL-6 Detection Antibody Concentrate (200X)
  • Human IL-6 Capture Antibody Concentrate (200X)
  • Human IL-6 Standard
  • Assay Diluent A (5X)
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Application

ELISA

Sensitivity
5.99 pg/mL
Standard Range
15.6-1000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 μL to 1,000 μL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials
  • Phosphate-Buffered Saline (PBS)

Antigen Details

Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Gene ID
3569 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/12/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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