Purified Rat IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK4530 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2b, κ
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
400601 50 µg $25.00
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400602 500 µg $110.00
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Description

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICFC, ICC, IHC, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin <  0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.

Application References

(PubMed link indicates BioLegend citation)
  1. Cervantes-Barragan L, et al. 2007. Blood 109:1131.
  2. Zeiser R, et al. 2007. Blood 109:2225.
  3. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
  4. Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
  5. Yi H, et al. 2009. Blood 113:5819. PubMed
  6. Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
  7. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
  8. Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Product Citations
  1. Matundan HH, et al. 2019. JCI Insight. 4:3. PubMed
  2. Alexander KA, et al. 2019. Front Immunol. 10:377. PubMed
  3. Lin D, et al. 2018. EMBO J. 37. PubMed
  4. Hwang BJ, et al. 2019. Oncogene. 38:7491. PubMed
  5. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  6. Atretkhany KN, et al. 2018. Proc Natl Acad Sci U S A. 115:13051. PubMed
  7. Yahata T, et al. 2019. Cancer Sci. 110:1279. PubMed
  8. Ray HC, et al. 2020. Sci Rep. 10:15808. PubMed
  9. Sasaki K, et al. 2007. Cancer Res. 67:6451. PubMed
  10. Sasaki K, et al. 2008. J Immunol. 181:104. PubMed
  11. Duan J, et al. 2008. Proc Natl Acad Sci U S A. 105:5183. PubMed
  12. Schaefer J, et al. 2009. J Leukoc Biol. 87:301. PubMed
  13. Yi H, et al. 2009. Blood. 113:5819. PubMed
  14. Lei G, et al. 2015. Infect Immun . 83:572. PubMed
  15. Richards J, et al. 2015. J Mol Cell Cardiol. 79:21. PubMed
  16. Moyat M, et al. 2015. Infect Immun . 83: 4217 - 4228. PubMed
  17. Saranchova I, et al. 2016. Sci Rep. 6: 30555. PubMed
  18. Vackova J, et al. 2020. Int J Mol Sci. 21:00. PubMed
  19. Coden ME, et al. 2020. J Immunol. 204:438. PubMed
  20. Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed
  21. Steffen E, et al. 2020. Basic Res Cardiol. 18:115. PubMed
  22. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed

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Go To Top Version: 2    Revision Date: 01/21/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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