Purified Rat IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK2758 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RTK2758_GoChIP_IgG2a_Iso-Con_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-Bcl11b (Clone 25B6, Cat. No. 650603) or B) equal amount of Go-ChIP-Grade™ Purified Rat IgG2a, κ Isotype Control Antibody (Clone RTK2758, Cat. No. 400569) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • RTK2758_GoChIP_IgG2a_Iso-Con_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-Bcl11b (Clone 25B6, Cat. No. 650603) or B) equal amount of Go-ChIP-Grade™ Purified Rat IgG2a, κ Isotype Control Antibody (Clone RTK2758, Cat. No. 400569) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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400501 50 µg $45.00
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400502 500 µg $120.00
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Description

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Reactivity
KLH
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ChIP - Verified
ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimoto H, et al. 2005. Blood 106:4241.
  2. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
  3. Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations
  1. Matsumoto K, et al. 2017. PLoS One. 12(6):e0175406. PubMed
  2. Oleinika K, et al. 2018. Nat Commun. 9:684. PubMed
  3. Murashov MD, et al. 2018. Pharmaceutics. 10:. PubMed
  4. Sharma MD, et al. 2018. Immunity. 48:91. PubMed
  5. Souriant S, et al. 2019. Cell Rep. 26:3586. PubMed
  6. Lopes N, et al. 2017. EMBO Mol Med. 0.954861111. PubMed
  7. Lever JM, et al. 2019. JCI Insight. 4:e125503. PubMed
  8. Itoh A, et al. 2019. Front Immunol. 2.198611111. PubMed
  9. Ray HC, et al. 2020. Sci Rep. 10:15808. PubMed
  10. Seach N, et al. 2008. J Immunol. 180:5384. PubMed
  11. Gopinath A, et al. 2020. J Immunol Methods. 476:112686. PubMed
  12. Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed
  13. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  14. Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed
  15. Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed
  16. Park GY, et al. 2020. Immune Netw. e43:20. PubMed
  17. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  18. Palau V, et al. 2021. Int J Mol Sci. 22: . PubMed
RRID
AB_326523 (BioLegend Cat. No. 400501)

Related FAQs

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Go To Top Version: 2    Revision Date: 08/24/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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