Purified Rat IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK2071 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
400401 50 µg $25.00
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400402 500 µg $90.00
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Description

The isotype of RTK2071 immunoglobulin is rat IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The IgG1, κ isotype control solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICFC, ICC, IHC, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this rat IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Application Notes

The RTK2071 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400414) as negative control1. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400432) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Riemann M, et al. 2005. J. Immunol. 175:3560. PubMed
  2. Wondimu Z, et al. 2010. Am. J. Pathol. 177:2334. PubMed
Product Citations
  1. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  2. Uehara H, et al. 2021. eLife. 10:00. PubMed
  3. Oleinika K, et al. 2018. Nat Commun. 9:684. PubMed
  4. Wan Jun Gan et al. 2018. Cell reports. 24(11):2819-2826 . PubMed
  5. Osborne SE, et al. 2017. Cell Microbiol. 19:3. PubMed
  6. Wichaiyo S, et al. 2019. Haematologica. 104:1648. PubMed
  7. Chiche A et al. 2017. Cell stem cell. 20(3):407-414 . PubMed
  8. Ondigo BN, et al. 2018. Am J Trop Med Hyg. 98:1770. PubMed
  9. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  10. Lagarrigue F, et al. 2018. Blood Adv. 1.720833333. PubMed
  11. Riemann M, et al. 2005. J Immunol. 175:3560. PubMed
  12. Pedras-Vasconcelos J, et al. 2008. J Immunol. 180:8231. PubMed
  13. Wondimu Z, et al. 2010. Am J Pathol. 177:2334. PubMed
RRID
AB_326507 (BioLegend Cat. No. 400401)
AB_326508 (BioLegend Cat. No. 400402)

Antigen Details

Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
Rat IgG1, kappa Isotype Ctrl
Specificity Alt (DOES NOT SHOW ON TDS):
IgG1, κ Isotype Ctrl
UniProt
View information about Rat IgG1, kappa Isotype Ctrl on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 01/22/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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