Purified Mouse IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MG1-45 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse (BALB/c) IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
MG1-45_GoChIP_IgG1_Iso-Con_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC1 (Clone 10E2/HDAC1, Cat. No. 815103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401409) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MG1-45_GoChIP_IgG1_Iso-Con_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC1 (Clone 10E2/HDAC1, Cat. No. 815103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401409) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
Cat # Size Price Quantity Avail. Save
401401 50 µg $40.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
401402 500 µg $110.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The isotype of MG1-45 immunoglobulin is mouse IgG1, κ. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
ChIP - Verified
WB, IP, ICC, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

The MG1-45 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401404) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 401408) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Ozeki M, et al. 2008. J. Leukocyte Biol. 84:769. PubMed
  2. Kraetzel K, et al. 2008. Eur. Respair. J. 32:563. PubMed
  3. de Jong MA, et al. 2008. J Gen Virol. 89:2398. PubMed
  4. Romee R, et al. 2012 Blood. 120:4751. PubMed.
  5. Wong-Baeza I, et al. 2013. J. Immunol. 190:3216. PubMed.
Product Citations
  1. Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed
  2. Espinosa-Díez C, et al. 2018. Redox Biol. 14:88. PubMed
  3. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
  4. Seoane M, et al. 2019. Oncogene. 38:3616. PubMed
  5. Cao Y, et al. 2019. Nat Commun. 10:1280. PubMed
  6. Staessens S, et al. 2019. Haematologica. 104(10). PubMed
  7. Camilli G, et al. 2018. Eur J Immunol. 48:757. PubMed
  8. Sun Z, et al. 2017. J Physiol. 595:1987. PubMed
  9. Fielding CA et al. 2017. eLife. 6 pii: e22206. PubMed
  10. Wülfing C, et al. 2018. Immun Inflamm Dis. 0.495833333. PubMed
  11. Vermi W, et al. 2018. Cancer Res. 78:3544. PubMed
  12. Bradford BM, et al. 2018. Parasite Immunol. 40:e12566. PubMed
  13. Kauer J, et al. 2019. Sci Rep. 9:18337. PubMed
  14. Uttagomol J, et al. 2019. Int J Mol Sci. 5.153472222. PubMed
  15. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  16. Wahlig S, et al. 2020. Sci Rep. 1.584027778. PubMed
  17. Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
  18. Satchwell TJ, et al. 2019. Nat Commun. 10:3806. PubMed
  19. Frasca D, et al. 2020. Front Immunol. 11:392. PubMed
  20. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  21. Sithole N, et al. 2020. Retrovirology. 17:6. PubMed
  22. Rolfes V, et al. 2020. Cell Reports. 31(6):107615. PubMed
  23. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  24. Meyrath M, et al. 2020. Nat Commun. 2.564583333. PubMed
  25. Parashar D, et al. 2019. Cell Rep. 29:4389. PubMed
  26. Kimura T, et al. 2020. Commun Biol. 0.527777778. PubMed
  27. Huang CH, et al. 2019. ACS Nano. 13:11698. PubMed
  28. Jong M, et al. 2008. J Gen Virol. 89:2398. PubMed
  29. Shively M 2008. J Leukoc Biol. 84:769. PubMed
  30. Kraetzel K, et al. 2008. Eur Respir J. 32:563. PubMed
  31. Evesson F, et al. 2010. J Biol Chem. 285:28529. PubMed
  32. Ji Y, et al. 2012. J Biol Chem. 287:24378. PubMed
  33. Romee R, et al. 2012. Blood. 120:4751. PubMed
  34. Wong-Baeza I, et al. 2013. J Immunol. 190:3216. PubMed
  35. Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed
  36. Barzilai S, et al. 2016. J Leukoc Biol. 99: 1045 - 1055. PubMed
  37. Wang F, et al. 2020. Int J Oncol. 57:707. PubMed
  38. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  39. Gabaev I, et al. 2020. Cell Rep. 33:108249. PubMed
  40. Essig F, et al. 2020. Int J Mol Sci. 21:00. PubMed
  41. Camilli G, et al. 2020. J Clin Invest. 130:4561. PubMed
  42. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  43. Maleki KT, et al. 2021. Cell Reports Medicine. 2(3):100220. PubMed
  44. Han N, et al. 2021. STAR Protocols. 2(2):100460. PubMed
RRID
AB_2801452 (BioLegend Cat. No. 401401)
AB_2801451 (BioLegend Cat. No. 401402)

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/24/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account