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Purified anti-Tata Binding Protein Antibody

Pricing & Availability
Clone
1TBP18 (See other available formats)
Regulatory Status
RUO
Other Names
TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit, TBP
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1TBP18_Purified_TataBindingProtein_Antibody_1_110817
Total cell lysates (15 µg protein) from HeLa, Jurkat and NIH3T3 were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL), 1:2000 diluted (0.25 µg/mL) and 1:5000 diluted (0.1 µg/mL) purified anti-Tata Binding Protein (clone 1TBP18) antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). M: protein marker.
  • 1TBP18_Purified_TataBindingProtein_Antibody_1_110817
    Total cell lysates (15 µg protein) from HeLa, Jurkat and NIH3T3 were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL), 1:2000 diluted (0.25 µg/mL) and 1:5000 diluted (0.1 µg/mL) purified anti-Tata Binding Protein (clone 1TBP18) antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). M: protein marker.
  • 1TBP18_Purified_TataBindingProtein_Antibody_2_050318
    Total lysates (15 µg protein) from 293T and 293T/Tata Binding Protein (TBP) CRISPR/Cas9 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-Tata Binding Protein antibody, clone 1TBP18 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 649203) was used as a loading control (lower). Lane M: MW ladder.
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668305 25 µg $101
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668306 100 µg $253
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Description

The TATA-binding protein, also known as TBP or Tata Binding Protein, is the general transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), which plays a role in the activation of eukaryotic genes transcribed by RNA polymerase II. This protein is a component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription. The rate of PIC formation is assumed to be primarily dependent on the rate of the association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: gel supershift.

 

Human TBP has 339 amino acid with predicted Mw 38 kD, while mouse TBP has 316 amino acid with predicted Mw 35 kD. Clone 1TBP18 recognizes epitope 1-20.

Application References

(PubMed link indicates BioLegend citation)
  1. Thompson NE, et al. 2004. Protein Expr. Purif. 36:186.
Product Citations
  1. Wang N, et al. 2020. Am J Physiol Cell Physiol. 319:C922. PubMed
  2. Phelps CA, et al. 2019. Sci Rep. 9:7990. PubMed
RRID
AB_2721754 (BioLegend Cat. No. 668305)
AB_2721755 (BioLegend Cat. No. 668306)

Antigen Details

Structure
TBP is a 37 kD transcription factor that is involved in eukaryotic transcription initiation.
Distribution

Nucleus

Function
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Interaction
Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3, SPIB, SNAPC1, SNAPC2 and SNAPC4, UTF1, BRF2, UBTF, GPBP1, HIV-1 Tat, herpes simplex virus 1 ICP4, TBP-TATA complex, LLPH, HSF1.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Hoffmann A, et al. 1990. Nature. 346:387.
  2. Peterson MG, et al. 1990. Science. 248:1625.
  3. Kwon H, et al. 1994. J Biol Chem. 269:30140.
  4. Comai L, et al. 1994. Science. 266:1966.
Gene ID
8148 View all products for this Gene ID
UniProt
View information about Tata Binding Protein on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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