Purified anti-T-bet Antibody

Pricing & Availability
Clone
4B10 (See other available formats)
Regulatory Status
RUO
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
1_4B10_PURE_T-Bet_Antibody_1_WB_103116.JPG
Total cell lysate from PBMC (lane 1, 15 µg) and PBMC treated with 5 µg/mL CD3 and 2 µg/mL CD28 (lane 2, 15 µg) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with purified anti-T-bet antibody (clone 4B10). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 1_4B10_PURE_T-Bet_Antibody_1_WB_103116.JPG
    Total cell lysate from PBMC (lane 1, 15 µg) and PBMC treated with 5 µg/mL CD3 and 2 µg/mL CD28 (lane 2, 15 µg) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with purified anti-T-bet antibody (clone 4B10). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 2_4B10_PURE_T-Bet_Antibody_2_ICFC_080619
    Human peripheral blood lymphocytes were surface stained with CD3 (clone OKT3) APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with purified T-bet (clone 4B10) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG1 PE.
  • 3_4B10_PURE_T-Bet_Antibody_WB_2_081417
    Total lysates (15µg protein) from Daudi, K562 and NK-92 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti- T-bet antibody (4B10, upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Check Availability Save
644801 25 µg $85.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
644802 100 µg $185.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB, ICFC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting and intracelluar immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For Western blotting, the suggested use is 1.0 to 2.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a staining volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References

(PubMed link indicates BioLegend citation)
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Wang D, et al. 2018. Immunity. 48:659. PubMed
  2. Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed
  3. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  4. Yang K, et al. 2017. Nature. 548:602. PubMed
  5. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  6. Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
  7. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  8. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  9. Seifert HA, et al. 2018. Metab Brain Dis. 33:1599. PubMed
  10. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  11. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  12. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  13. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  14. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  15. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  16. Lee B, et al. 2019. Front Immunol. 0.561805556. PubMed
  17. Hsieh C, et al. 2012. J Pharmacol Exp Ther. 343:125. PubMed
  18. Stankovic S, et al. 2015. J Immunol. 195: 4892 - 4899. PubMed
  19. Chen J, et al. 2020. J Immunol. 204:2076. PubMed
  20. Wang Y, et al. 2021. Cancer Cell. :. PubMed
  21. Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
RRID
AB_1595608 (BioLegend Cat. No. 644801)
AB_1595503 (BioLegend Cat. No. 644802)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 08/06/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account