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Purified anti-rat IL-6 Antibody

Pricing & Availability
Clone
Poly5177 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-6 (IL-6), Interferon beta 2 (IFNB2), BSF2, HGF, HSF
Isotype
Goat Polyclonal IgG
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Poly5177_Rat_IL-6_Antibody_082213
  • Poly5177_Rat_IL-6_Antibody_082213
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517701 100 µg $350
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Description

Interleukin 6 (IL-6) is a prototypic member of the IL-6 superfamily of cytokines. IL-6 is secreted by a variety of lymphoid and non-lymphoid cells including T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, keratinocytes, etc. Mature Rat IL-6 is a 187 aa residue protein. At the protein sequence level, there is approximately 39% identity between rat and human IL-6, and 87% identity between mouse and rat IL-6. The production of IL-6 can be induced by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines, and viruses.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a subunit (gp130) that acts as the universal signal-transducing element for all IL-6 family cytokines. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. One of the most important mechanism is that IL-6 is a key factor that reciprocally regulates Th17 and Foxp3+ Treg differentiation by inhibition of TGF-β induced Foxp3 and induction of RORγt, a Th17 lineage-specific transcription factor.

Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections, trauma, autoimmune diseases, inflammations and malignancies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For sandwich ELISA capture applications, a concentration range of 1-2 μg/ml is recommended when used in conjunction with the biotinylated Poly5177 antibody (Cat. No. 517703) as the detecting antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Poly5177 is 4.1% cross-reactive with mouse IL-6 as measured by ELISA.

RRID
AB_2561337 (BioLegend Cat. No. 517701)

Antigen Details

Structure
187 amino acids (aa) in length, and 39% and 87% aa sequence identity with human and mouse IL-6, respectively.
Distribution
Secreted by a variety of lymphoid and non-lymphoid cells including T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, keratinocytes and muscle cells, etc.
Function
A pleiotropic cytokine that plays important roles in innate and adaptive immunity. Also important for hematopoiesis, bone metabolism, and cancer progression.
Ligand/Receptor
IL-6 receptor
Cell Type
B cells, Dendritic cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Heinrich PC, et al. 1990. Biochem. J. 265:621.
2. Hibi M, et al. 1996. J. Mol. Med. 74:1.
3. Hirano T, et al. 1994. Stem Cells 12:262.
4. Taga T and Kishimoto T. et al. 1997. Annu. Rev. Immunol. 15:797.
5. Van Snick J. 1990. Annu. Rev. Immunol. 8:253.

Regulation
Expression can be induced by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines, and viruses.
Gene ID
24498 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All IL-6 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-rat IL-6 Poly5177 ELISA Capture
Biotin anti-rat IL-6 Poly5177 ELISA Detection
Go To Top Version: 2    Revision Date: 02/09/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-rat IL-6

    Poly5177_Rat_IL-6_Antibody_082213

  • Biotin anti-rat IL-6

    Poly5177_Rat_IL-6_Antibody_082213
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