- 3H5 (See other available formats)
- Regulatory Status
- Other Names
- B7-1, B7, BB1, Ly-53
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD80 is a 60 kD member of the immunoglobulin superfamily also known as B7-1, B7, and BB1. It is expressed on antigen presenting cells and activated T and B cells, and is a ligand for CD28 and CD152 (CTLA-4). CD80/CD28 interactions are important for T and B cell co-stimulation, while CD80/CD152 lead to the inhibition of T cell responses. The 3H5 antibody can block the co-stimulatory activity of CD80.Product Details
- Antibody Type
- Host Species
- HTLV-1-transformed Lewis-S1 rat T cell line
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of ligand binding1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200208).
(PubMed link indicates BioLegend citation)
- Product Citations
AB_313843 (BioLegend Cat. No. 200202)
- Ig superfamily, 60 kD
Antigen presenting cells, activated B cells and T cells
- T and B cell costimulation
- CD28, CD152 (CTLA-4)
- Cell Type
- Antigen-presenting cells, B cells, T cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Linsley, P.S., et al. 1991. J. Exp. Med. 174:561.
3. Salomon, B., et al. 2001. Ann. Rev. Immunol. 19:225.
- Gene ID
- 25408 View all products for this Gene ID
- View information about CD80 on UniProt.org
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