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Purified anti-RAP1 Antibody

Pricing & Availability
Clone
W18011A (See other available formats)
Regulatory Status
RUO
Other Names
TERF2IP, TERF2 Interacting Protein, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric protein 1, Repressor/activator protein 1 homolog, Dopamine receptor-interacting protein 5, DRIP5
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W18011A_PURE_RAP1_Antibody_1_072720
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-RAP1 antibody (clone W18011A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18011A_PURE_RAP1_Antibody_1_072720
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-RAP1 antibody (clone W18011A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18011A_PURE_RAP1_Antibody_2_072720
    Whole cell extracts (15 µg total protein) from Raji lysates were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-RAP1 antibody (clone W18011A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18011A_PURE_RAP1_Antibody_3_072720
    Whole cell extracts (250 µg total protein) prepared from Raji cells were immunoprecipitated overnight with 2.0 µg of purified rat IgG2b, κ isotype control antibody (Cat. No. 400602) (panel A) or purified anti-RAP1 antibody (clone W18011A) (panel B). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody against a separate epitope of RAP1. Lane M: Molecular weight marker.
  • W18011A_PURE_RAP1_Antibody_4_072720
    Raji cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5 µg/mL (1:100 dilution) of either purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400602) (panel A) or purified anti-RAP1 antibody (clone W18011A) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL (1:250 dilution). Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
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938301 25 µg $101
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938302 100 µg $253
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Description

Repressor/activator protein 1 (RAP1), also called telomeric repeat-binding factor 2-interacting protein 1 (TERF2IP), is one of six subunits of the shelterin complex, which regulates telomerase activity and protects telomeric DNA through the generation of t-loops. RAP1 forms a heterodimer with shelterin subunit TRF2 and regulates telomere length, recombination, and transcription. This complex also functions in the repression of homology-directed repair (HDR) and prevents telomere deletions. In the cytoplasm, RAP1 functions as an inhibitor kinase (IKK) adaptor and modulator of the nuclear factor- κB (NF-κB) pathway as well as an activator of NF-κB transcriptional activity. TERF2IP S205 phosphorylation has been implicated in the regulation of activation, apoptosis, and senescence of endothelial cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human RAP1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize mouse RAP1.

ICC testing was conducted on Raji cells fixed with 4% paraformaldehyde and permeabilized with either Triton X-100 or methanol.  Both methods were compatible with RAP1 staining.

RRID
AB_2876760 (BioLegend Cat. No. 938301)
AB_2876760 (BioLegend Cat. No. 938302)

Antigen Details

Structure
RAP1 is a 399 amino acid protein with an expected molecular weight of 55 kD.
Distribution

Ubiquitously expressed/Nuclear

Function
Transcription, Transcription regulation
Biology Area
Cell Biology, DNA Repair/Replication
Antigen References
  1. Cai Y, et al. 2017. Cell Cycle. 16:1765-1773.
  2. Kotla S, et al. 2019. JCI Insight. 4(9):124867.
  3. Rai R, et al. 2015. Nat Commun. 7:10881.
  4. Zhang Y, et al. 2015. Cell Death Discov. 1:15007.
Gene ID
54386 View all products for this Gene ID
UniProt
View information about RAP1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/27/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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