- W15221A (See other available formats)
- Regulatory Status
- Other Names
- Nuclear factor of activated T-cells 3, NFAT4, NFATX
- Rat IgG2a, κ
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- Product Citations
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Nuclear factor of activated T cells C3 (NFATC3), also known as NFAT4 or NFATx, is a member of the NFAT family of transcription factors. Several NFATC3 variants generated by alternative splicing were identified. NFATC3 plays an important role in the regulation of T cell activation-related gene expression, especially in the induction of IL-2. NFATC3 is expressed in immune cells, such as double positive (DP) thymocytes, mature CD4+ T cells, and mast cells. It is also found in non-immune cells such as smooth muscle and Schwann cells. In the resting T cells, NFATC3 is located in the cytoplasm and is phosphorylated. Upon T cell activation by TCR stimulation, the increase of intracellular Ca2+ induces NFATC3 dephosphorylation via calcineurin. Dephosphorylated NFATC3 exposes the nuclear localization signal and is subsequently translocated from the cytosol to the nucleus. Through the highly conserved Rel-like binding domain, NFATC3 associates with specific consensus DNA sequences and regulates gene expression.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Partial human NFATC3 recombinant protein (826-1075 a.a.) expressed in E. coli.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone W15221A may react with isoforms 1-4 (MW 112.6~115.6). NFATs are heavily phosphorylated proteins, so the observed MWs of NFATC3 can be higher than predicted.
AB_2629823 (BioLegend Cat. No. 690602)
- There are 6 reported isoforms of NFATC3 (MWs: 115.1, 115.2, 115.6, 112.6, 77.2, and 80.6 kD). The protein contains a RHD (Rel-like) domain responsible for DNA binding.
Cytoplasm and nucleus.
- NFATC3 is a component of activated T cells DNA-binding transcription complex, regulating gene expression in immature thymocytes and T cells.
- Forms a multicomponent complex with NFATC1 and NFATC2. Interacts with Raf-1.
- Biology Area
- Cell Biology, Transcription Factors
- Antigen References
1. Ran Y, et al. 2014. J. Recept. Signal. Transduct. Res. 34:270.
2. Geil WM, et al. 2014. FEBS J. 281:1170.
3. Neria F, et al. 2013. Glia. 61:1052.
4. Urso K, et al. 2011. Blood. 118:795.
5. Canté-Barrett K, et al. 2007. J. Immunol. 179:103.
6. Chen J, et al. 2003. J. Immunol. 170:3109.
- Gene ID
- 4775 View all products for this Gene ID
- View information about NFATC3 on UniProt.org
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