Purified anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
H57-597_Pure_071608.jpg
C57L/6 splenocytes stained with H57-597 purified, followed by anti-Armenian hamster IgG FITC
  • H57-597_Pure_071608.jpg
    C57L/6 splenocytes stained with H57-597 purified, followed by anti-Armenian hamster IgG FITC
  • H57-597_pure_TCR-Beta_Antibody_IHC-F_102518
    Fresh, frozen mouse spleen was stained with purified TCR β chain clone H57-597 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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109201 50 µg $45.00
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109202 500 µg $115.00
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F  - Verified
IP, Costim, Depletion - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  2. Tan A, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.004. PubMed
  3. Lin J, et al. 2017. Nat Commun. . 10.1038/s41467-017-01477-5. PubMed
  4. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  5. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  6. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  7. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  8. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  9. Wang HC, et al. 2017. J Immunol. 198:3149. PubMed
  10. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  11. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  12. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  13. Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed
  14. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  15. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  16. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
  17. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  18. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  19. Clark K, et al. 2019. Front Immunol. 10:2355. PubMed
  20. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  21. Sharba S, et al. 2019. Virulence. 10:97. PubMed
  22. Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed
  23. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  24. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  25. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  26. Lee B, et al. 2019. Front Immunol. 0.561805556. PubMed
  27. Tsukumo S, et al. 2006. J Immunol. 177:8365. PubMed
  28. Chappaz S, et al. 2007. Blood. 110:3862. PubMed
  29. McDole J, et al. 2010. Am J Pathol. 177:1823. PubMed
  30. Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed
  31. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
RRID
AB_313424 (BioLegend Cat. No. 109201)
AB_313425 (BioLegend Cat. No. 109202)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 08/09/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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