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Purified anti-mouse/rat β-Amyloid, 1-16 Antibody (Previously Covance catalog# SIG-39155)

Pricing & Availability
Clone
M3.2 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Covance Catalog# SIG-39155
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M3-2_PUREAF_beta-Amyloid_041018
Western blot of purified anti-mouse/rat β-Amyloid, 1-16 antibody (clone M3.2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain total lysate; Lane 4: 20ug of rat brain total lysate. The blot was incubated with 0.025 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • M3-2_PUREAF_beta-Amyloid_041018
    Western blot of purified anti-mouse/rat β-Amyloid, 1-16 antibody (clone M3.2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain total lysate; Lane 4: 20ug of rat brain total lysate. The blot was incubated with 0.025 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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805707 25 µL $112
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805701 200 µL $546
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805702 1 mL $1805
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Description

Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The epitope has been determined to be within residues 10-15 of murine Aβ.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC, IP, ELISA Detection, ELISA Capture - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.005 - 0.025 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone M3.2 recognizes a rodent sequence of Aß, ß-CTF, sAPP-a, and full length APP. Clone 3.2 has negligible cross reactivity with human isoforms of APP and beta amyloid.

Application References

(PubMed link indicates BioLegend citation)
  1. Morales-Corraliza J, et al.  2009. PLoS One 4(9):e7134. (IP, WB, ELISA Detection)
  2. Teich AF, et al. 2013. PLoS One 8(2):e55647, 2013. (ELISA Capture, WB)
  3. Lee Y, et al. 2017. Neuropharmacology. 117:387-400. (ICC)
  4. Lundgren JL, et al. 2015. J Neurochemistry. 135:606-15. (ICC)
Product Citations
  1. Joly S, et al. 2021. Int J Mol Sci. 22:. PubMed
  2. Miranda AM, et al. 2018. Nat Commun. 9:291. PubMed
  3. Sun J, et al. 2019. Nat Commun. 10:53. PubMed
  4. Daria A, et al. 2017. EMBO J. 10.15252/embj.201694591. PubMed
  5. Tambini MD, et al. 2020. Elife. 9:e52612. PubMed
  6. Socodato R, et al. 2020. Cell Reports. 31(12):107796. PubMed
  7. Gulisano W, et al. 2019. J Neurosci. 39:5986. PubMed
  8. Wang W et al. 2019. EBioMedicine. 42:174-187 . PubMed
RRID
AB_2734556 (BioLegend Cat. No. 805707)
AB_2564982 (BioLegend Cat. No. 805701)
AB_2564981 (BioLegend Cat. No. 805702)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue sources: Primarily nervous system, but also adipose tissue, intestine, muscle.

Distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1.
  3. Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
  4. Puig KL, Combs CK. 2013. Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ, Hardy J. 2016. EMBO Mol. Med. 8(6):595.
  6. Walsh DM, Selkoe DJ. 2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-16 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 03/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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