Purified anti-mouse IL-27Rα Antibody

Pricing & Availability
Clone
W16125D (See other available formats)
Regulatory Status
RUO
Other Names
interleukin27 receptor alpha, IL27ra, IL-27Ra, TCCR, WSX-1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
W16125D_PURE_IL-27Ra_Antibody_1_061020
C57BL/6 splenocytes were stained with CD3 APC and purified IL-27Rα (clone W16125D) (left) or purified rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE.
  • W16125D_PURE_IL-27Ra_Antibody_1_061020
    C57BL/6 splenocytes were stained with CD3 APC and purified IL-27Rα (clone W16125D) (left) or purified rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE.
  • W16125D_PURE_IL-27Ra_Antibody_2_061020
    C57BL/6 splenocytes were stained with CD130 purified (followed by anti-rat IgG2b FITC) and purified IL-27Rα (clone W16125D) (left) or purified rat IgG2a, κ isotype control (right), followed by anti-rat IgG2a PE.
Cat # Size Price Quantity Check Availability Save
159001 25 µg $90.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
159002 100 µg $280.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

69 kD glycosylated transmembrane subunit of IL-27 receptor complex consisting of IL27Ra (WSX-1, TCCR) and gp130 (CD130). Expressed on T cells, monocytes, and neutrophils and aside from its effect on innate immunity, is involved in dampening the extent of Th1 immune response post infection. Activity levels of  INF-γ and IL-17 producing T cells are regulated through this receptor which relays signal through Jak-STAT pathway.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse IL-27Rα transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2860746 (BioLegend Cat. No. 159001)
AB_2860747 (BioLegend Cat. No. 159002)

Antigen Details

Distribution

IL27Ra is expressed by T cells, monocytes, and neutrophils.

Ligand/Receptor
IL27Ra combines with gp130 to form a receptor complex whose activation by IL-27 leads to differing pathway-dependent effector activities.
Cell Type
Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Takeda A, et al. 2003. J Immunol. 170:4886-90.
  2. Yoshida H & Hunter CA. 2015. Annu Rev Immunol. 33:417-43.
  3. Iwasaki Y, et al. 2015. Int J Mol Sci. 16:2851-63.
Gene ID
50931 View all products for this Gene ID
UniProt
View information about IL-27Ralpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/10/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account