Purified anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
R4-6A2 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Avail. Save
505701 50 µg $70.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
505702 500 µg $115.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially-purified, native mouse IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture1-4,6,10,11 or ELISPOT Capture5: The purified R4-6A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated XMG1.2 antibody (Cat. No. 505804) as the detecting antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT Capture, the suggested use of this antibody is 1.0 - 4.0 µg/mL.
ELISA or ELISPOT Detection: The biotinylated R4-6A2 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified XMG1.2 antibody (Cat. Nos. 505802 & 505812) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard.
Neutralization1,2,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. Nos. 505708 - 505713).
Additional reported applications (for the relevant formats) include: immunohistochemical staining3,6,8 of acetone-fixed frozen tissue sections.
Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 and 430804) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Stevenson M, et al. 1990. Infect. Immun. 58:3225.
  3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
  4. Yang X, et al. 1993. J. Immunoassay 14:129.
  5. Klinman D, et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.
  6. Sander B, et al. 1991. Immunol. Rev. 119:65.
  7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
  8. Khanna A, et al. 2000. J. Immunol. 164:1346.
  9. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349.
  10. Dzhagalov I, et al. 2007. J. Immunol. 178:2113.
  11. Xu G, et al. 2007. J. Immunol. 179:5358.
Product Citations
  1. Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed
  2. Rauschenberger T, et al. 2019. Front Immunol. 10:1917. PubMed
  3. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  4. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  5. Xu G, et al. 2007. J Immunol. 179:5358. PubMed
  6. Lai L, et al. 2011. Cancer Res. 71:61. PubMed
  7. Singh R, et al. 2017. J Immunol. 10.4049/jimmunol.1602010. PubMed
RRID
AB_315389 (BioLegend Cat. No. 505701)
AB_315390 (BioLegend Cat. No. 505702)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
  2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
  3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
  4. Gray P, et al. 1987. Lymphokines 13:151.
Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/25/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account