Purified anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
H22 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Armenian Hamster IgG
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Product Citations
publications
H22_PURE_IFNgamma_Antibody_WB_050416
Western blot analysis of 50 ng recombinant human IFN-γ (carrier-free) (lane 1), recombinant rat IFN-γ (carrier-free) (lane 2), and recombinant mouse IFN-γ (carrier-free) (lane 3) using anti-mouse IFN-γ (clone H22) antibody followed by HRP goat anti-Armenian hamster IgG secondary antibody.
  • H22_PURE_IFNgamma_Antibody_WB_050416
    Western blot analysis of 50 ng recombinant human IFN-γ (carrier-free) (lane 1), recombinant rat IFN-γ (carrier-free) (lane 2), and recombinant mouse IFN-γ (carrier-free) (lane 3) using anti-mouse IFN-γ (clone H22) antibody followed by HRP goat anti-Armenian hamster IgG secondary antibody.
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513202 100 µg $85.00
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Recombinant full-length murine IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ELISA, ICC, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified H22 antibody is useful in mouse IFN-γ ELISA, immunofluorescence and immunoprecipitation applications. For in vitro or in vivo neutralization applications, it is suggested to use LEAF™ Purified H22. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Schreiber RD, et al. 1985. J. Immunol. 134:1609.
  2. Williams RO, et al. 1993. Clin. Exp. Immunol. 92:323
  3. Steed AL, et al. 2006. J. Virol. 80:192.
  4. Wu LL, et al. 2014. Am J Pathol. 184:2260. PubMed
Product Citations
  1. Wu L, et al. 2014. Am J Pathol. 184:2260. PubMed
RRID
AB_1089144 (BioLegend Cat. No. 513202)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Function
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571
4. Gray P, et al. 1987. Lymphokines 13:151

Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 01/18/2021

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