Purified anti-mouse CD55 (DAF) Antibody

Pricing & Availability
Clone
RIKO-3 (See other available formats)
Regulatory Status
RUO
Other Names
Decay accelerating factor (DAF)
Isotype
Armenian Hamster IgG
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Product Citations
publications
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131802 100 µg $150
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Description

CD55, also known as DAF (decay accelerating factor), is expressed on the surfaces of red blood cells, hematopoietic cells and many non-hemotopietic cells. There are two isoforms of mouse CD55, a GPI-anchored isofrom and a transmembrane isoform. Both are recognized by RIKO-3. The function of CD55 is to protect damage by complement deposition.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Cao Q, et al. 2012. Am J Physiol Cell Physiol. 302:1731. PubMed.
Product Citations
  1. Olcina MM et al. 2018. Cell reports. 25(13):3721-3732 . PubMed
  2. Cao Q, et al. 2012. Am J Physiol Cell Physiol. 302:C1731. PubMed
RRID
AB_1279269 (BioLegend Cat. No. 131802)

Antigen Details

Structure
60-70 kd single chain glycoprotein. While human CD55 contains only one CD55 gene, two distinct CD55 genes have been identified in the mouse, referred to as GPI-DAF and transmembrane(TM)-DAF. GPI-DAF is expressed only in testis.
Distribution

Red blood cells and most of the splenocytes, including B cells and T cells, and also many non-hematopoetic cells.

Function
Protect from complement attack by inhibition the deposition of C3 on antibody-sensitized cells.
Cell Type
B cells, Erythrocytes, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Miwa T et al. 2001. Immunology 104:207
2. Lin FY et al. 2001 Immunology 104:215

Gene ID
13136 View all products for this Gene ID
UniProt
View information about CD55 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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