- SA15-21 (See other available formats)
- Regulatory Status
- Other Names
- Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells, and T cells. TLR4 has been recognized as critical for host recognition of LPS in conjunction with MD2.Product Details
- Antibody Type
- Host Species
- LPS-stimulated TLR4/MD-2/CD14 cotransfected NKR cells.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1 Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.
Additional reported applications (for the relevant formats) include: immunoprecipitation1 and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).
(PubMed link indicates BioLegend citation)
- Akashi S, et al. 2003. J. Exp. Med. 198:1035. (IP)
- Wakabayashi Y, et al. 2006. J. Immunol. 177:1772. (Block)
- Visintin A, et al. 2006. J. Leukoc. Biol. 80:1584. (FC)
- Shibata T, et al. 2011. Int. Immunol. 23:503. (FC)
- Product Citations
AB_2561871 (BioLegend Cat. No. 145401)
AB_2561872 (BioLegend Cat. No. 145402)
- Type I transmembrane protein complex
- Macrophages, B cells and T cells
- Activates the innate immune system against Gram-negative pathogens by recognition of LPS
- Bacterial LPS
- Cell Type
- Macrophages, B cells, T cells, Dendritic cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Toll Like Receptors
- Antigen References
1. Brennan TV, et al. 2012. Blood 120:2899.
2. Wantia N, et al. 2011. PLoS One 6:e26101.
3. Loser K, et al. 2010. Nat. Med. 16:713.
4. Kang S, et al. 2009. Blood 113:2605.
- Gene ID
- 21898 View all products for this Gene ID
- View information about CD284 on UniProt.org
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