- 7D4/CD25 (See other available formats)
- Regulatory Status
- Other Names
- IL-2Rα, IL2RA, IL-2Ra, Ly-43, p55, Tac
- Rat IgM, κ
- Ave. Rating
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- Product Citations
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CD25, also known as IL-2 low affinity receptor is a type I, 55 kDa, transmembrane glycoprotein. CD25 It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.Product Details
- Antibody Type
- Host Species
- HT-2 cell line
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
Block, Depletion, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Inhibition of IL-2 dependent proliferation of HT2 cells, immunoprecipitation of CD25 from cell lysates, in vivo depletion of CD25+ cells.
(PubMed link indicates BioLegend citation)
- Malek TR, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:5694. (FC, Block, IP)
- Couper KN. 2007. J. Immunol. 178:4136. (Depletion)
- Product Citations
AB_2716173 (BioLegend Cat. No. 154202)
- Type I, 55 kD, transmembrane glycoprotein
Activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Huss DJ, et al. 2015. J. Immunol. 194:84.
2. Hartmann FJ, et al. 2014. Nat. Commun. 5:5056.
3. Codarri L, et al. 2007. J. Exp. Med. 204:1533.
4. Read S, et al. 2000. J. Exp. Med. 192:295.
5. Taniguchi T, et al. 1993. Cell 73:5.
- Gene ID
- 3559 View all products for this Gene ID
- View information about CD25 on UniProt.org
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