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Purified anti-mouse CD147 Antibody

Pricing & Availability
Clone
OX-114 (See other available formats)
Regulatory Status
RUO
Other Names
Basigin
Isotype
Rat IgG1, κ
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Product Citations
publications
OX-114_PURE_CD147_Antibody_IHC-F_1_020818.png
C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse CD147 (clone OX-114) purified overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG (clone Poly4053) Alexa Fluor® 647 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured by 10X objective.
  • OX-114_PURE_CD147_Antibody_IHC-F_1_020818.png
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse CD147 (clone OX-114) purified overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG (clone Poly4053) Alexa Fluor® 647 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured by 10X objective.
  • OX-114_PURE_CD147_Antibody_IHC-F_2_020818.png
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse CD147 (clone OX-114) purified overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG (clone Poly4053) Alexa Fluor® 647 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured by 10X objective.
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123701 50 µg $65
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Description

CD147 is a widely expressed membrane glycoprotein and reported to be implicated in cell adhesion, embryonic development, T cell activation and many other functions. It is expressed in thymocytes and many other cells, including epithelial and endothelial cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone OX-114 has been shown to bind both two and three domain forms of mouse CD1471.

Application References

(PubMed link indicates BioLegend citation)
  1. Hanna SM, et al. 2003. BMC Biochemistry 4:17.
RRID
AB_1227519 (BioLegend Cat. No. 123701)

Antigen Details

Structure
Single chain type I transmembrane 58 kd heavily glycosylated protein. N terminal extracellular part consists of two C2 type Ig-like domains which are highly glycosylated. Recently reported: isoform with an additional membrane-distal Ig-like domain.
Distribution

CD147 is expressed at varying levels in many cells types, including haematopoietic, epithelial, and endothelial cells. Expressed on immature thymocytes and at varying levels on spleen cells , bone marrow cells and macrophages.

Function
CD147 is implicated in multiple functions, such as matrix metalloproteinase induction, cell adhesion, retinal cell development, HIV attachment, embryonic development and T cell activation.
Ligand/Receptor
cyclophilin
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hanna SM, et al. 2003. BMC Biochemistry 4:17
2. Yurchenko V, et al. 2005. Immunology 117:301
3. Renno T, et al. 2002. J. Immunol. 168:4946

Gene ID
12215 View all products for this Gene ID
UniProt
View information about CD147 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/17/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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