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Purified anti-Lck Antibody

Pricing & Availability
Clone
LCK-01 (See other available formats)
Regulatory Status
RUO
Other Names
Proto-oncogene tyrosine kinase lck, p56-Lck, T-cell specific protein tyrosine kinase
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_LCK-01_Purified_Lck_Antibody_031519.png
Total cell lysates (15 µg total protein) from Jurkat, MOLT4, EL4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-LCK Antibody, clone LCK-01, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_LCK-01_Purified_Lck_Antibody_031519.png
    Total cell lysates (15 µg total protein) from Jurkat, MOLT4, EL4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-LCK Antibody, clone LCK-01, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 2_LCK-01_PURE_LCK_Antibody_1_WB_072616
    Total cell lysate from Jurkat cells (lane 1, 15 µg), mouse thymus (lane 2, 15 µg) and HeLa cells (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-Lck antibody (clone LCK-01). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 3_LCK-01_PURE_Lck_Antibody_2_IF_090916
    Jurkat cells were fixed with 4% PFA for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were stained with 2 µg/mL anti-Lck Antibody (clone LCK-01) followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG in blocking buffer for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 4_LCK-01_Purified_Lck_Antibody_IP_100219
    Whole cell extracts (300 µg total protein) prepared from Jurkat cells were immunoprecipitated overnight with 2.5 µg of Purified mouse IgG1, κ Isotype Control Antibody (Cat. No. 401401) or Purified anti-Lck Antibody, clone LCK-01. The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1.0 µg/mL (1:500 dilution) of LCK-01. Lane M: Molecular Weight marker.
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628301 25 µg $106
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628302 100 µg $229
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Description

Lck is a 56 kD tyrosine protein kinase and a member of the src subfamily that contains both an SH2 and SH3 domain. Alternatively spliced isoforms of Lck have been reported. Lck is a cytoplasmic protein bound to CD4 and CD8 in T lymphocytes that participates in antigen-induced T cell activation through phosphorylation of the T cell receptor zeta chain. Lck is activated upon T cell receptor engagement and is involved in the onset of cell cycle progression induced by interleukin-2. Phosphorylation by Csk downregulates the activity of Lck. In addition to CD4 and CD8, Lck has been reported to interact with PI3K through its SH3 domain and tyrosine phosphorylated KHDRBS1/p70 through its SH2 domain. In addition, Lck has been shown to associate with the SAP transmembrane tyrosine phosphatase. The Lck-01 monoclonal antibody recognizes human Lck and has been shown to be useful for Western blotting, immunoprecipitation, immunocytochemistry, and flow cytometry.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide corresponding to a.a. 22-36 in human Lck
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
FC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Does not cross-react with mouse.

Clone may cross-react with other Src tyrosine kinase family members.

Application References

(PubMed link indicates BioLegend citation)
  1. Romagnoli P, et al. 2001. Int. Immunol. 13:305.
Product Citations
  1. Ghosh S, et al. 2020. Cell Reports. 30(10):3434-3447. PubMed
  2. Baumgart F, et al. 2016. Nat Methods. 10.1038/nmeth.3897. PubMed
  3. Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
RRID
AB_2136337 (BioLegend Cat. No. 628301)
AB_2136336 (BioLegend Cat. No. 628302)

Antigen Details

Structure
Belongs to the tyrosine family of kinases, src subfamily. Contains one SH2 domain, 1 SH3 domain. Approximately 56 kD, alternatively spliced isoforms have been reported
Distribution

Cytoplasmic protein, bound to cytoplasmic CD4 and CD8 in T lymphocytes

Function
Participates in antigen-induced T cell activation, phosphorylates zeta chain of T cell receptor. Involved in interleukin-2 induced onset of cell cycle progression
Interaction
Associates with CD4 and CD8 co-receptors. Binds to PI3K through SH3 interactions, binds to tyrosine phosphorylated KHDRBS1/p70 through SH2 domain. Interacts with SAP transmembrane tyrosine phosphatase
Modification
Phosphorylation
Cell Type
T cells
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Koga Y, et al. 1986. Eur. J. Immunol. 16:1643.
2. Vogel LB, et al. 1995. Biochem. Biophys. Acta 1264:168.
3. Vogel LB, et al. 1993. Mol. Cell. Biol. 13:7408.
4. Bergaman M, et al. 1992. EMBO J. 11:2919.

Regulation
Activated upon T cell receptor engagement. Tyrosine phosphorylation by p50 Csk downregulates catalytic activity
Gene ID
3932 View all products for this Gene ID
UniProt
View information about Lck on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 8    Revision Date: 10/02/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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