Purified anti-human VEGFR-3 (FLT-4) Antibody

Pricing & Availability
Clone
9D9F9 (See other available formats)
Regulatory Status
RUO
Other Names
Receptor tyrosine Kinase VEGFR-3, VEGFR3, FLT4
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
9D9F9_Purified_VEGFR3_Antibody_FC_010813
HUVEC, human endothelial cells were stained with purified VEGFR3 (clone 9D9F9) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 9D9F9_Purified_VEGFR3_Antibody_FC_010813
    HUVEC, human endothelial cells were stained with purified VEGFR3 (clone 9D9F9) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
Cat # Size Price Quantity Check Availability Save
356201 50 µg $105.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
356202 200 µg $285.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Receptor tyrosine Kinase VEGFR-3, also known as FLT4, together with VEGFR1 (FIT1) and VEGFR2 (KDR/Flk-1), are the receptors for vascular endothelial growth factors (VEGF). The VEGFR family belongs to the class II subfamily of receptor tyrosine kinases (RTKs), containing a large extracellular region which is composed of seven Ig-like domains (D1–D7), a single transmembrane, helix, and a cytoplasmic region with tyrosine kinase activity. In VEGFR-3, the fifth Ig homology domain is proteolytically cleaved which results in polypeptides that remain linked by two disulfide bonds. VEGFR-3 is widely expressed on all endothelial cells in early embryogenesis, while, in adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. VEGF-C and VEGF-D activation of VEGFR-3 plays an important role in the formation of the lymphatic vessel system. Aberrant activation or expression of VEGFR and their ligands has been implicated in tumor angiogenesis, coronary artery disease, diabetic blindness, and other diseases.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
VEGFR extracellular domain protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: immunohistochemistry staining of frozen or paraffin embedded sections1-3.

Application References

(PubMed link indicates BioLegend citation)
  1. Jussila L, et al. 1998. Cancer Res. 58:1599. (IHC)
  2. Kilic N, et al. 2007. Blood 110:4223. (IHC)
  3. Folpe A, et al. 2000. Mod. Pathol. 13:180. (IHC)
Product Citations
  1. Cooley L, et al. 2016. Sci Rep. 6: 25840. PubMed
  2. Karlas A, et al. 2016. Nat Commun. 7:11320. PubMed
RRID
AB_2561770 (BioLegend Cat. No. 356201)
AB_2561771 (BioLegend Cat. No. 356202)

Antigen Details

Structure
Seven Ig-like domains in the extracellular domain, a single transmembrane helix, and a cytoplasmic region with tyrosine kinase activity
Distribution

Widely expressed in early embryogenesis endothelial cells, restrictly expressed on lymphatic endothelial cells in adults

Function
Mediates lymphangiogenesis in response to VEGF-C and VEGF-D, involved in cardiovascular development during embryogenesis
Ligand/Receptor
VEGF-C, VEGF-D
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Tammela T, et al. 2008. Nature 454:656.
2. Partanen TA, et al. 2000. FASEB J. 14:2087.
3. Valtola R, et al. 1999. Am. J. Pathol. 154:1381.
4. Jussila L, et al. 1998. Cancer Res. 58:1599.
5. Galland F, et al. 1992. Genomics 13:475.

Gene ID
2324 View all products for this Gene ID
UniProt
View information about VEGFR-3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/22/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account