- GoH3 (See other available formats)
- Regulatory Status
- HCDM listed
- Other Names
- VLA-6 α chain, α6 integrin, integrin α6, ITGA6
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, and epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.Product Details
- Human, African Green, Mouse, Baboon, Capuchin Monkey, Cat (Feline), Cattle (Bovine, Cow), Chimpanzee, Cynomolgus, Dog (Canine), Horse (Equine), Rabbit (Lapine), Rhesus, Sheep (Ovine), Swine (Pig, Porcine)
- Antibody Type
- Host Species
- Mouse mammary tumor cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
ICC - Verified
IHC-F, IP, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin a61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin a6 function in vivo.
(PubMed link indicates BioLegend citation)
- Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
- Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
- Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
- Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
- Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
- Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
- Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
- Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
- Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
- Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
- Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
- Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
- Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
- Product Citations
AB_345296 (BioLegend Cat. No. 313602)
- Integrin family, associates with β1 or β4, 120 kD
Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells
- Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
- With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
- Cell Type
- Embryonic Stem Cells, Endothelial cells, Epithelial cells, Monocytes, Platelets, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.
- Gene ID
- 16403 View all products for this Gene ID 3655 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Specificity Alt (DOES NOT SHOW ON TDS):
- View information about CD49f on UniProt.org
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