Purified anti-human IL-9 Antibody

Pricing & Availability
Clone
MH9A4 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
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507602 500 µg $240.00
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Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 has also been reported to protect human T cells from apoptosis induced by IL-2 withdrawal. IL-9 is upregulated in human eosinophils by TNF-α and IL1-β. IL-9 has been reported to downregulate the oxidative burst in activated human alveolar macropahges and induce TGF-β production. The MH9A4 antibody reacts with human IL-9. The MH9A4 antibody can neutralize the bioactivity of natural or recombinant IL-9.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Baculovirus-expressed, recombinant human IL-9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-9 recombinant protein ranging from 1000 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture2: The purified MH9A4 antibody is useful as the capture antibody in a human IL-9 sandwich ELISA assay, when used in conjunction with the biotinylated MH9D1 antibody as the detecting antibody.
For human IL-9 neutralization assay, the LEAF™ purified MH9D1 antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-9 bioactivity.

Application References

(PubMed link indicates BioLegend citation)
  1. Jenmalm M, et al. 2001. Clin. Exptl. Aller. 31:1528.
  2. Faulkner H, et al. 2002. J. Infec. Diseas. 185:665.
  3. Chen J, et al. 2008. Blood 111:5163. PubMed
  4. Chang HC, et al. 2010. Nat. Immunol. 11:527. (ELISA) PubMed
  5. Lozano E, et al. 2012. J Immunol. 188:3869. PubMed.
Product Citations
  1. Chen J, et al. 2008. Blood. 111:5163. PubMed
  2. Lozano E, et al. 2012. J Immunol. 188:3678. PubMed
RRID
AB_315484 (BioLegend Cat. No. 507602)

Antigen Details

Structure
Cytokine; 32-39 kD (Mammalian)
Bioactivity
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources
IL-2 activated TH2 lymphocytes, Hodgkin's lymphoma
Cell Targets
T lymphocytes, erythroid precursors
Receptors
IL-9R
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y. 1992. Leuk. Lymphoma 8:441.

Regulation
Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID
3578 View all products for this Gene ID
UniProt
View information about IL-9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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