Purified anti-human IgD Antibody

Pricing & Availability
Clone
W18340A (See other available formats)
Regulatory Status
RUO
Other Names
Ig delta chain C region
Isotype
Rat IgG2a, κ
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Product Citations
publications
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human IgD (clone W18340A) at 4°C overnight. On the next day, tissue was incubated with goat anti-rat IgG (Poly4054) Alexa Fluor® 594 antibody (red) and anti-human CD19 (clone A17136C) Alexa Fluor® 647 antibody (green) at room temperature for two hours. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
  • Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human IgD (clone W18340A) at 4°C overnight. On the next day, tissue was incubated with goat anti-rat IgG (Poly4054) Alexa Fluor® 594 antibody (red) and anti-human CD19 (clone A17136C) Alexa Fluor® 647 antibody (green) at room temperature for two hours. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
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324502 100 µg $45.00
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Description

IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length human IgD recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2888899 (BioLegend Cat. No. 324502)

Antigen Details

Structure
Exists in a transmembranal and a soluble form
Distribution

Naïve B cells

Function
Antigen binding, B cell activation
Interaction
The CD79a/CD79b heterodimer
Molecular Family
Immune Checkpoint Receptors, Innate Immune Signaling
Antigen References
  1. Geisberger R, et al. 2006. Immunology 118:429-37.
  2. Weller S, et al. 2005. Eur J Immunol. 35:2789-92.
  3. Brandtzaeg P & Johansen FE. 2005. Immunol Rev. 206:32-63.
Gene ID
3495 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/12/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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