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Purified anti-human CD95 (Fas) (Maxpar® Ready) Antibody

Pricing & Availability
Clone
DX2 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-64
Other Names
Fas, APO-1, TNFRSF6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DX2_Purified_CD95_Cytof_061014.jpg
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 164Dγ-anti-CD95 [FAS] (DX2). Data provided by DVS Sciences.
  • DX2_Purified_CD95_Cytof_061014.jpg
    Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 164Dγ-anti-CD95 [FAS] (DX2). Data provided by DVS Sciences.
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305631 100 µg $211
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Description

CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CD95 transfected L cells
Formulation
Phosphate-buffered solution, pH7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656).

Note: EOS9.1 antibody can induce apoptosis without cross-linking.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
  3. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop)
  4. Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC)
  5. Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC)
  6. Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  10. Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed
Product Citations
  1. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
RRID
AB_2563766 (BioLegend Cat. No. 305631)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 45 kD
Distribution

T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes

Function
Mediates apoptosis
Ligand/Receptor
Fas ligand (CD178)
Cell Type
B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449.

Gene ID
355 View all products for this Gene ID
UniProt
View information about CD95 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD95 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD95 (Fas) DX2 FC
Biotin anti-human CD95 (Fas) DX2 FC
FITC anti-human CD95 (Fas) DX2 FC
PE anti-human CD95 (Fas) DX2 FC
PE/Cyanine5 anti-human CD95 (Fas) DX2 FC
Purified anti-human CD95 (Fas) DX2 FC,ICC,IHC
Alexa Fluor® 488 anti-human CD95 (Fas) DX2 FC
Alexa Fluor® 647 anti-human CD95 (Fas) DX2 FC
Brilliant Violet 421™ anti-human CD95 (Fas) DX2 FC
Pacific Blue™ anti-human CD95 (Fas) DX2 FC
PE/Cyanine7 anti-human CD95 (Fas) DX2 FC
Brilliant Violet 605™ anti-human CD95 (Fas) DX2 FC
PerCP/Cyanine5.5 anti-human CD95 (Fas) DX2 FC
Purified anti-human CD95 (Fas) (Maxpar® Ready) DX2 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD95 (Fas) DX2 FC
APC/Fire™ 750 anti-human CD95 (Fas) DX2 FC
APC/Cyanine7 anti-human CD95 (Fas) DX2 FC
Brilliant Violet 510™ anti-human CD95 (Fas) DX2 FC
Brilliant Violet 711™ anti-human CD95 (Fas) DX2 FC
Brilliant Violet 785™ anti-human CD95 (Fas) DX2 FC
Brilliant Violet 650™ anti-human CD95 (Fas) DX2 FC
Alexa Fluor® 700 anti-human CD95 (Fas) DX2 FC
TotalSeq™-A0156 anti-human CD95 (Fas) DX2 PG
TotalSeq™-C0156 anti-human CD95 (Fas) DX2 PG
TotalSeq™-B0156 anti-human CD95 (Fas) DX2 PG
Ultra-LEAF™ Purified anti-human CD95 (Fas) DX2 FC,ICC,IHC,Apop
TotalSeq™-D0156 anti-human CD95 (Fas) DX2 PG
PE/Fire™ 640 anti-human CD95 (Fas) DX2 FC
KIRAVIA Blue 520™ anti-human CD95 (Fas) DX2 FC
APC/Fire™ 810 anti-human CD95 (Fas) Antibody DX2 FC
PE anti-human CD95 DX2 FC
Spark YG™ 593 anti-human CD95 (Fas) DX2 FC
PE/Fire™ 700 anti-human CD95 (Fas) DX2 FC
PerCP/Fire™ 780 anti-human CD95 (Fas) DX2 FC
Spark Blue™ 574 anti-human CD95 (Fas) DX2 FC
Go To Top Version: 2    Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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