Purified anti-human CD95 (Fas) (Maxpar® Ready) Antibody

Pricing & Availability
Clone
DX2 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-64
Other Names
Fas, APO-1, TNFRSF6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DX2_Purified_CD95_Cytof_061014.jpg
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 164Dγ-anti-CD95 [FAS] (DX2). Data provided by DVS Sciences.
  • DX2_Purified_CD95_Cytof_061014.jpg
    Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 164Dγ-anti-CD95 [FAS] (DX2). Data provided by DVS Sciences.
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305631 100 µg $180.00
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Description

CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CD95 transfected L cells
Formulation
Phosphate-buffered solution, pH7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. The product is formulated to simplify the antibody preparation when performing the labeling protocol. As a result, it is possible to proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656).

Note: EOS9.1 antibody can induce apoptosis without cross-linking.

Additional Product Notes

Maxpar® is a registered trademark of Fluidigm Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
  3. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop)
  4. Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC)
  5. Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC)
  6. Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  10. Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed
Product Citations
  1. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
RRID
AB_2563766 (BioLegend Cat. No. 305631)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 45 kD
Distribution

T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes

Function
Mediates apoptosis
Ligand/Receptor
Fas ligand (CD178)
Cell Type
B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449.

Gene ID
355 View all products for this Gene ID
UniProt
View information about CD95 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 1    Revision Date: 08/08/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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