Purified anti-human CD88 (C5aR) Antibody

Pricing & Availability
Clone
S5/1 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
S5slash1_PE_092809
Human peripheral blood granulocytes stained with S5/1 PE
  • S5slash1_PE_092809
    Human peripheral blood granulocytes stained with S5/1 PE
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344302 100 µg $110.00
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Description

CD88 is a single chain protein with seven membrane-spanning regions and has a MW of 43 kD. CD88 is expressed by monocytes, neutrophils and eosinophils. Also, there have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as Gi, and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules, resulting in chemotaxis, granule enzyme release and superoxide anion production.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant peptide huC5aR N-terminal -NT (Asp15-Asp27)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S5/1 blocks the binding of C5a to CD88.

Application References

(PubMed link indicates BioLegend citation)
  1. Kiener HP, et al. 1998. Arthritis Rheum. 41:233.
  2. Elsner J, et al. 1994. Blood 83:3324.
  3. Oppermann M, et al. 1993. J. Immunol. 151:3785.
Product Citations
  1. Colley CS, et al. 2018. MAbs. 10:104. PubMed
  2. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  3. Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
  4. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
  5. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
  6. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
RRID
AB_2259318 (BioLegend Cat. No. 344302)

Antigen Details

Structure
CD88 is a single chain protein with seven membrane-spanning regions and a MW of 43 kD.
Distribution

CD88 is expressed by monocytes, neutrophils and eosinophils. There has been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes and vascular endothelial cells.

Function
After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction
CD88 is coupled to heterotrimeric G proteins such as Gi. The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
Cell Type
Eosinophils, Monocytes, Neutrophils
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol Chem. 284:12363.
3. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
4. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
5. Buck E and Wells JA. et al. 2005. P. Natl. Acad. Sci. USA 102:2719.

Gene ID
728 View all products for this Gene ID
UniProt
View information about CD88 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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