Purified anti-human CD51 Antibody

Pricing & Availability
Clone
NKI-M9 (See other available formats)
Regulatory Status
RUO
Workshop
IV 103
Other Names
Vitronectin receptor α chain, Integrin αV, αV integrin, ITGAV
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
NKI-M9_Pure_020808
Human melanoma cell line M21 stained with purified NKI-M9, followed by anti-mouse IgG FITC
  • NKI-M9_Pure_020808
    Human melanoma cell line M21 stained with purified NKI-M9, followed by anti-mouse IgG FITC
  • NKI-M9_Purified_CD51_Antibody_ICC_081619
    MDA-MB231 breast cancer cell line was stained with anti-human CD51, detected with anti-mouse DyLight™ 649, and nuclear counterstained with DAPI. Images were acquired with a TE300 fluorescence microscope with a 20x objective. Data provided by: Er Liu and John Nolan, La Jolla Bioengineering Institute
Cat # Size Price Quantity Check Availability Save
327902 100 µg $100.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD51 is a type I integral membrane glycoprotein, known as vitronectin receptor α chain, or integrin αV. It forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD, and is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling, mediates bone absorption and angiogenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Melonama cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
ELISA, Block, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Defilippi P, et al. 1991. J. Cell Biol. 114:855. PubMed
  3. Burdick MM, et al. 2003. Am. J. Physiol.Cell Ph. 284:C977. PubMed
  4. Grzeszkiewicz TM, et al. 2001. J. Biol. Chem. 276:21943.
  5. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145. PubMed
  6. Silverman AP, et al. 2008. J Mol Biol. 385:1064. PubMed
  7. Khurana S, et al. 2013. Blood. 121:2587. PubMed
Product Citations
  1. John Lin CC, et al. 2017. Nat Neurosci. 1.108333333. PubMed
RRID
AB_2129615 (BioLegend Cat. No. 327902)

Antigen Details

Structure
Integrin, forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8, 125 kD and 24 kD in reduced condition, 150 kD (unreduced)
Distribution

Endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.

Function
Adhesion, angiogenesis
Ligand/Receptor
Vitronectin, von Willebrand factor, fibrinogen, thrombospondin, osteopontin, denatured collagen, fibronectin, laminin.
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Mesenchymal Stem Cells, Osteoclasts, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Nesbitt S, et al. 1993. J. Biol. Chem. 268:16737.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication

Gene ID
3685 View all products for this Gene ID
UniProt
View information about CD51 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/16/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account